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Citations & References (5)
Invitrogen™
Pro-Q™ Diamond Phosphoprotein Blot Stain Kit
The Pro-Q Diamond Phosphoprotein Blot Stain Kit provides a rapid and simple method for directly detecting phosphoproteins on polyvinylidene difluorideRead more
| Catalog Number | Quantity |
|---|---|
| P33356 | 1 kit |
Catalog number P33356
Price (CNY)
6,486.00
Each
Quantity:
1 kit
Price (CNY)
6,486.00
Each
The Pro-Q Diamond Phosphoprotein Blot Stain Kit provides a rapid and simple method for directly detecting phosphoproteins on polyvinylidene difluoride (PVDF) or nitrocellulose membranes. The fluorescent stain binds selectively to the phosphate moiety and binding is independent of the amino acid sequence context. Because it is a direct stain, you don't need expensive antibodies or radioisotopes. The Pro-Q Diamond Phosphoprotein Blot Stain Kit can be used to analyze the native phosphorylation state of proteins obtained from tissue specimens or from body fluids.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Blot Detection
Detection MethodFluorescence
Product LinePRO-Q
Product TypePhosphoprotein Blot Stain Kit
Quantity1 kit
Shelf Life6 Months
Target MoleculeProteins (Phosphoproteins)
Label or DyePro-Q Diamond
Unit SizeEach
Citations & References (5)
Citations & References
Abstract
The human mitochondrial transcription termination factor (mTERF) is fully active in vitro in the non-phosphorylated form.
Journal:J Biol Chem
PubMed ID:15899902
'The human mitochondrial transcription termination factor (mTERF) is a 39-kDa protein that terminates transcription at the 3''-end of the 16 S rRNA gene and thereby controls expression of the ribosomal transcription unit of mitochondrial DNA. The transcription termination activity of human mTERF has been notoriously difficult to study in vitro,
Ca(2+)-related signaling and protein phosphorylation abnormalities play central roles in a new experimental model of electrical storm.
Journal:Circulation
PubMed ID:21555709
Electrical storm (ES), characterized by recurrent ventricular tachycardia/fibrillation, typically occurs in implantable cardioverter-defibrillator patients and adversely affects prognosis. However, the underlying molecular basis is poorly understood. In the present study, we report a new experimental model featuring repetitive episodes of implantable cardioverter-defibrillator firing for recurrent ventricular fibrillation (VF), in which
Pin1 promotes histone H1 dephosphorylation and stabilizes its binding to chromatin.
Journal:
PubMed ID:24100296
Histone H1 plays a crucial role in stabilizing higher order chromatin structure. Transcriptional activation, DNA replication, and chromosome condensation all require changes in chromatin structure and are correlated with the phosphorylation of histone H1. In this study, we describe a novel interaction between Pin1, a phosphorylation-specific prolyl isomerase, and phosphorylated
Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using a novel fluorescent phosphorylation sensor dye.
Journal:Proteomics
PubMed ID:12872225
Ultrasensitive detection of minute amounts of phosphorylated proteins and peptides is a key requirement for unraveling many of the most important signal transduction pathways in mammalian systems. Protein microarrays are potentially useful tools for sensitive screening of global protein expression and post-translational modifications, such as phosphorylation. However, the analysis of
Detection of phosphoproteins on electroblot membranes using a small-molecule organic fluorophore.
Journal:Electrophoresis
PubMed ID:15300773
A new formulation of the small-molecule organic fluorophore, Pro-Q Diamond dye, has been developed that permits rapid and simple detection of phosphoproteins directly on polyvinylidene difluoride (PVDF) or nitrocellulose membranes (electroblots). Protein samples are first separated by electrophoresis and then electroblotted to membranes, stained and destained, in an analogous manner