Fluorescein-Ubiquitin
Fluorescein-Ubiquitin
Citations & References (1)

Fluorescein-Ubiquitin

Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due toRead more
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Catalog NumberQuantity
PV437750 μg
PV4378500 μg
Catalog number PV4377
Price (CNY)
4,646.00
Each
Add to cart
Quantity:
50 μg
Price (CNY)
4,646.00
Each
Add to cart
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission340⁄490, 520
For Use With (Equipment)Microplate Reader
Label TypeClassic Dyes
Label or DyeFITC (Fluorescein)
Quantity50 μg
ReadoutEnd Point
Shipping ConditionDry Ice
Substrate PropertiesProtein-Based Substrate
TechniqueTR-FRET
ConjugateFluorescein
FormLiquid
Unit SizeEach
Contents & Storage
Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.

Citations & References (1)

Citations & References
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Authors:Madiraju C, Welsh K, Cuddy MP, Godoi PH, Pass I, Ngo T, Vasile S, Sergienko EA, Diaz P, Matsuzawa S, Reed JC,
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation, ... More