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Citations & References (1)
Invitrogen™
Biotin-Ubiquitin
Biotin-labeled ubiquitin for use as a general research tool, or in high throughput assays to monitor protein ubiquitination. Due toRead more
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| Catalog Number | Quantity |
|---|---|
| PV4379 | 10 μg |
| PV4380 | 100 μg |
Catalog number PV4379
Price (CNY)
4,613.00
Each
Quantity:
10 μg
Price (CNY)
4,613.00
Each
Biotin-labeled ubiquitin for use as a general research tool, or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.
For use in combination with other LanthaScreen™ products including LanthaScreen™ Tb-Ubiquitin (Cat. No. PV4375), Fluorescein-Ubiquitin (PV4377), or a LanthaScreen™ antibody such as Tb-anti-biotin (PV4749).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission340/490, 520 nm
For Use With (Equipment)Microplate Reader
Label TypeBiotin & Other Haptens
Label or DyeBiotin
Quantity10 μg
ReadoutEnd Point
Shipping ConditionDry Ice
Substrate PropertiesProtein-Based Substrate
TechniqueTR-FRET
ConjugateBiotin
FormLiquid
Unit SizeEach
Contents & Storage
Biotin labeled ubiquitin in HEPES buffered saline. Store at -20°C.
Citations & References (1)
Citations & References
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation,