Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
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Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Invitrogen™

Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits

Green features
Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.
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Catalog NumberQuantityAssayQuantitation Range
Q33265100 assays1X dsDNA, broad range4 to 4000 ng
Q33230100 assays1X dsDNA, high sensitivity0.1 to 120 ng
Q33231500 assays1X dsDNA, high sensitivity0.1 to 120 ng
Q33266500 assays1X dsDNA, broad range4 to 4000 ng
Catalog number Q33265
Price (CNY)
1,454.00
Each
Add to cart
Quantity:
100 assays
Assay:
1X dsDNA, broad range
Quantitation Range:
4 to 4000 ng
Price (CNY)
1,454.00
Each
Add to cart
Experience easy, accurate, and precise quantification of dsDNA with Qubit 1X dsDNA HS (High Sensitivity) and Qubit 1X dsDNA BR (Broad Range) Assay Kits. The 1X formulation provides a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometers. These assays are highly selective for dsDNA over ssDNA, RNA, protein, and free nucleotides. Contaminants, such as salts, solvents, or detergents are well-tolerated.
Qubit 1X dsDNA HS and BR Assay Kits, designed for use with Qubit Fluorometers, are highly selective for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA), RNA, protein, and free nucleotides. All kits provide a ready-to-use working solution and DNA standards. To perform the assay, simply dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a Qubit Fluorometer.

Qubit 1X dsDNA HS Assay Kit
The Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 5 pg/μL to 120 ng/μL, providing a detection range of 0.1−120 ng.

Qubit 1X dsDNA BR Assay Kit
The Qubit dsDNA BR (Broad-Range) Assay Kit, designed for use with Qubit 4 or Qubit Flex Fluorometers, provides an accurate and selective method for the quantitation of DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 4,000 ng/μL, providing a detection range of 4-4,000 ng.

Notes
• Qubit 1X dsDNA HS can be used with Qubit 2, Qubit 3, Qubit 4, and Qubit Flex Fluorometers
• Qubit 1X dsDNA BR are designed for use with Qubit 4 and Qubit Flex Fluorometers
• Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200 μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer

Specifications
Assay1X dsDNA, broad range
For Use With (Equipment)Qubit 4 and Qubit Flex Fluorometer
Green FeaturesFewer resources used, Less waste
No. of Reactions100 Reactions
Product LineQubit
Quantitation Range4 to 4000 ng
Quantity100 assays
Shipping ConditionThis assay kit will ship as two components.
• Qubit™ 1X dsDNA BR Working Solution will be shipped (at ambient conditions.)
• Qubit 1X dsDNA BR lambda standards will be shipped on wet ice.
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

Can the Qubit 1X dsDNA, broad range (BR) Assay Kit (Cat. No. Q33265) be used on the Qubit 3 Fluorometer?

No, this assay is only compatible with Qubit 4 Fluorometer (Cat. No. Q33226) and Qubit Flex Fluorometer (Cat. No. Q33327).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

What is the shelf life for the 1X dsDNA BR working solution(Cat. No. Q33262), that is part of the Qubit 1X dsDNA BR Assay (Cat Nos. Q33265, Q33266)?

The storage conditions for the 1X dsDNA BR working solution are 18–28 degrees C (room temperature). When stored as directed, the kit contents are stable for at least 6 months from delivery date. The standards should still be kept in the fridge.

After mixing the sample or the standard with the working solution, the fluorescence signal is stable for 3 hours at room temperature when samples are protected from light, following the incubation period.
Storage information can be found on page 1, and incubation time is on page 2, of the Qubit 1X dsDNA BR Assay User Guide

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

I would like to use my Qubit 4 Fluorometer with the Qubit 1X dsDNA BR Assay, but the assay is not listed as an option on the fluorometer. How can I resolve this?

The Qubit 4 Fluorometer needs to be updated. The Qubit 4 Fluorometer can be updated with the USB that was provided with the Qubit. If you do not have the USB, we recommend using an empty 2.0GB that is FAT32 formatted. If the USB is not recognized, leave USB plugged in for a few minutes before running the update. This should give you the assay you need to run the Qubit Assay.

You can find the updates on the Technical Resources for Qubit Fluorometers page.

Does the Qubit Flex Quantitation Starter Kit (Cat. No. Q45894) include a Qubit dsDNA Broad Range Assay Kit (Cat. No. Q33265, Q33266)?

No. The Qubit Flex Quantitation Starter Kit (Cat. No. Q45894) does not include a Qubit dsDNA Broad Range Assay Kit (Cat. No. Q33265, Q33266). It must be purchased separately.

The description of the Qubit Flex Starter Packages (Cat. Nos. Q32894, Q45893, Q45894) can be found on the Qubit Flex Starter Packages product page.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

What is the expiration date for Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266)?

The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266) are stable for 6 months from the date of receipt when stored as directed.

For more information on product storage, please refer to the user guides:

Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.