DNA Loading Dye & SDS Solution (6X)

Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependent nucleases.

Highlights

• 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
• 100 mM EDTA inhibits metal-dependent nucleases

Applications

• Analysis of DNA samples with high amounts of DNA binding proteins
• Kinetic experiments
• DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation