ALN Disc

Differentiate Fusobacterium species from Bacteroides species using Thermo Scientific™ Remel™ ALN Kit. ALN Kit is recommended for use in detection of the enzyme L-alanyl-L-alanyl aminopeptidase in bacterial cells. Detection of this enzyme is a rapid, cost-effective procedure for screening cultures by presumptively placing organisms in the appropriate genus (Bacteroides spp. are ALN-positive, while Fusobacterium spp. are ALN-negative).

The extent of anaerobe identification is a concern to laboratories due to the time and expense involved with some methods^1,2. Anaerobe isolates from sites of serious infections and normally sterile body sites should be definitively characterized. However, presumptive grouping and preliminary identification based on rapid techniques can provide clinically relevant information.

Use ALN Kit for detection of the enzyme, L-alanyl-L-alanyl aminopeptidase in bacterial cells. Detection of this enzyme is a rapid, cost-effective procedure for screening cultures by presumptively placing organisms in the appropriate genus.

• Easy to store - No special storage required
• Easy to interpret - With visual inspection

The ALN disk contains a substrate L-alanyl-L-alanyl- β-naphthylamide. Aminopeptidase hydrolyzes this substrate to release β-naphthylamine. Following the addition of the Cinnamaldehyde Reagent, β-naphthylamine reacts with ρ-dimethylaminocinnamaldehyde to form a red colored complex, indicating a positive result.