PRO Disk with Reagent

Identify yeast and anaerobic microorganisms, primarily Candida albicans and Clostridium difficile, using Thermo Scientific™ Remel™ PRO Kit. PRO Kit is recommended for use in qualitative procedures for detection of the enzyme L-Proline aminopeptidase in yeast and bacterial cells. The PRO Kit may be used as a single colony screening test to aid in the identification of Candida albicans and Clostridium difficile. The PRO disk is 99% positive when testing C. difficile from anaerobic cultures. It is useful in differentiating other Gram-positive and Gram-negative bacilli.

Use PRO Kit for identification of Candida albicans and Clostridium difficile. C. albicans is the yeast pathogen most frequently isolated from clinical specimens¹. In 1990, Perry et al. evaluated a rapid test to aid in the identification of C. albicans based on colorimetric detection of the enzyme, L-proline aminopeptidase². C. difficile is recognized as the major cause of antibiotic-associated pseudomembranous colitis and a frequent cause of hospital-acquired diarrhoea. Fedorko and Williams reported detection of L-Proline aminopeptidase is useful for identification of C. difficile because it is one of the few biochemical tests for which this organism is positive³.

• Easy to store - No special storage required
• Easy to read - After addition of one drop of Cinnamaldehyde a color change from red to pink color development indicates a positive test

The PRO disk contains the substrate L-proline-β-naphthylamide. This substrate is hydrolyzed by an aminopeptidase releasing β-naphthylamine. After addition of Cinnamaldehyde Reagent, β-naphthylamine reacts with ρ- dimethyl-aminocinnamaldehyde to form a red-colored complex.