Differentiate and identify Mycobacterium species based on their ability to reduce potassium tellurite using Thermo Scientific™ Remel™ 0.2% Potassium Tellurite. In 1969, Kilburn et al. described the differential identification of mycobacteria utilizing the tellurite reduction test1. Tween® 80 is added to Middlebrook 7H9 Broth to test for tellurite reduction. Certain species of mycobacteria reduce potassium tellurite at variable rates. Most rapid growers reduce tellurite in 3 days2.
Members of Mycobacterium avium are distinguished from most other nonphotochromogens by the ability to reduce tellurite in 3 days. The enzyme tellurite reductase reduces potassium tellurite, an electron acceptor, to metallic tellurite forming a black precipitate2.Ready-to-use - Liquid reagent.Easy to interpret -Formation of a black precipitate of metallic tellurium in and around the sedimented mycobacterial cells indicates a positive test.This reagent contains 0.2%w/v potassium tellurite supplied as 25 mL bottles.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
- Kilburn, J.O., V.A. Silcox, and G.P. Kubica. 1969. Am. Rev. Respir. Dis. 99:94.
- Kent, P.T. and G.P. Kubica. 1985. Public Health Mycobacteriology, A Guide for the Level III Laboratory. U.S. Dept. of H.H.S., CDC, Atlanta, GA.