Differentiate and identify Mycobacterium spp. with the catalase test using Thermo Scientific™ Remel™ Polysorbate 80, 10% reagent. Most strains of mycobacteria produce catalase1. The catalase test is a semiqualitative test which was developed to help in differentiation of organisms included in Mycobacterium tuberculosis complex (drug-resistant strains) from other Mycobacterium spp2 (non-drug-resistant strains)3,4.
Polysorbate 80 helps in dispersing the hydrophobic, tightly clumped mycobacterial cells from large aggregates to individual bacilli, thus maximizing the detection of catalase. 10% Polysorbate 80 permits more rapid entry of compounds, such as 30% hydrogen peroxide into the mycobacterial cell by lowering the surface tension in a medium.Ready-to-use: Liquid reagentEasy to interpret:Strong Positive Test: A column of bubbles > 45mmWeak Positive Test: A column of bubbles < 45mmNegative Test: No bubblesThis reagent contains 0.2 g/1000mL of Polysorbate 80 (CAS 9005-65-6) supplied in 10mL bottles.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
General References:
- Isenberg, H.D. 2004. Clinical Microbiology Procedures Handbook. 2nd ASM Press, Washington, D.C.
- Wayne, L.G. and J.R. Doubek. 1968. Appl. Microbiol. 16:925-931.
- Kent, P.T. and G.P. Kubica. Public Health Mycobacteriology, A Guide for the Level III Laboratory. 1985. U. S. Dept. of H.H.S., CDC, Atlanta, GA.
- Forbes, B.A., D.F. Sahm, and A.S. Weissfeld. 2007. Bailey and Scott's Diagnostic Microbiology. 12th Mosby Elsevier, St. Lois, MO.