Use 5% Oxalic Acid reagent to digest and decontaminate clinical specimens prior to inoculation of media for isolating Mycobacterium species.
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R21285
250 mL
Catalog number R21285
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Quantity:
250 mL
Digest and decontaminate clinical specimens prior to inoculation of media for isolating Mycobacterium species using Thermo Scientific™ Remel™ Oxalic Acid reagent. In 1930, Corper and Uyei described an oxalic acid procedure prior to inoculation of media for digestion and decontamination of clinical specimens1. They reported growth of acid-fast bacilli on a variety of media after using this reagent.
Most clinical specimens submitted to the microbiological laboratory for isolation of acid-fast bacilli are contaminated with more rapidly growing commensal microbial flora2. Acid-fast bacilli may also be trapped in respiratory secretions and cannot be cultured without liquefaction of the specimen3. Due to its high acid content, Oxalic Acid (5%) acts as a decontaminating agent. It also acts as a liquefying agent to release the mycobacterial cells from mucin in the respiratory specimen.Ready-to-use: Liquid reagentEspecially useful in specimens contaminted with Pseudomonas spp3.This reagent contains 5% w/v of oxalic acid supplied as 250mlL bottles.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Corper, H.J. and N.Uyei. 1930. J. Lab. Clin. Med. 15:348-369.
Kubica, G.P., A.J. Kaufmann, and W.E. Dye. 1963. Amer. Rev. Resp. Dis. 89:284-286.
Kent, P.T. and G.P. Kubica. 1985. Public Health Mycobacteriology, A Guide for the Level III Laboratory. U. S. Dept. of H.H.S., CDC, Atlanta, GA.