Mycobiotic Agar (MycoTube) with Chloramphenicol, Cycloheximide

Selectively isolate and cultivate pathogenic fungi from clinical specimens which may be contaminated with bacteria and saprophytic fungi using Thermo Scientific™ Remel™ Mycobiotic Agar (MycoTube) with chloramphenicol, cycloheximide. The use of cycloheximide as a selective agent for inhibition of saprophytic fungi was first described by Leach, Ford, and Whitten¹. In 1958, Huppert and Walker added cycloheximide and chloramphenicol to mycological agar for isolation of Coccidioides immitis². The agar came to be known as Mycobiotic Agar³.

Mycobiotic Agar is a solid medium used for selective cultivation of fungal pathogens from diverse clinical samples and other materials contaminated with a mixed associated flora. It is recommended for recovery of dimorphic fungi and dermatophytes from clinical specimens potentially contaminated with bacteria and saprophytic fungi^4,5.

• Ready to use: Convenience of prepared media.
• Selective: Cycloheximide and chloramphenicol are selective agents that inhibit saprophytic fungi and bacteria.

This medium contains soy peptone which provides nitrogen, amino acids, and peptides necessary for the growth of fungi and dextrose as a source of energy which supports the growth of fungi. Cylcoheximide inhibits rapidly growing saprophytic fungi that may overgrow slower-growing pathogens. Chloramphenicol is a broad-spectrum antibiotic which inhibits gram-positive and gram-negtaive bacteria, as well as Nocardia spp.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.