Quant-it™ RiboGreen Reagent and RNA Assay Kit
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Quant-it™ RiboGreen Reagent and RNA Assay Kit
Invitrogen™

Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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Catalog NumberQuantityProduct TypeQuantitation Range
R327001 kitRediPlate 96 RiboGreen RNA Kit3 to 200 ng
R114901 kitQuant-iT RiboGreen RNA Kit1 to 200 ng
R114911 mLQuant-iT RiboGreen RNA Reagent1 to 200 ng
Catalog number R32700
Price (CNY)
2,503.00
飞享价
Ends: 31-Dec-2025
3,163.00
Save 660.00 (21%)
Each
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Quantity:
1 kit
Product Type:
RediPlate 96 RiboGreen RNA Kit
Quantitation Range:
3 to 200 ng
Price (CNY)
2,503.00
飞享价
Ends: 31-Dec-2025
3,163.00
Save 660.00 (21%)
Each
Add to cart
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

Workflow 

This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection.  To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL.  RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.

Applications

Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
Product LineRIBOGREEN, RediPlate
Product TypeRediPlate 96 RiboGreen RNA Kit
Quantitation Range3 to 200 ng
Quantity1 kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

When I try to run RediPlate 96 RiboGreen RNA Kit (Cat. No. R32700) on my Varioskan LUX instrument, it says there is no plate. What should I do?

When using a strip plate with the Varioskan LUX plate reader, ensure that there is a strip covering the D6 well, as this is where the instrument checks for the presence of a plate. The strip does not need to be active; it just needs to be in place for the instrument to verify the plate's presence. Alternatively, you can resolve this issue by adjusting the software settings. Go to the general settings and uncheck the box labeled "Check plate and tip prime vessel before session execution." This will immediately solve the problem.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does the Quant-it RiboGreen RNA Assay Kit and RiboGreen RNA Reagent (Cat. No. R32700) work with double-stranded RNA (dsRNA)?

We have not validated measurement of dsRNA with the Quant-it RiboGreen RNA Assay Kit and RiboGreen RNA Reagent (Cat. No. R32700). If you are using RiboGreen dye to measure dsRNA, then you should use your own dsRNA standards, rather than the standards provided with the kit, to get an accurate measurement.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center