Lactophenol Aniline Blue

Microscopically examine fungi in wet mount preparations using Thermo Scientific™ Remel™ Lactophenol Aniline Blue. Linder recommended lactophenol as a mounting fluid for examination and preservation of fungi¹. He reported that lactophenol kills organisms without causing cytoplasmic plasmolysis. Lactophenol Aniline Blue is recommended by Koneman, Roberts, and Wright for direct mounting and staining of fungi.²

Lactophenol Aniline Blue is a stain recommended for use in wet mount preparations for direct mounting and staining of fungi.

• Ready to use: Liquid reagent
• Easy identification: Yeast cells, mycelia and fruiting structures stain blue and are visible under a microscope

This reagent contains phenol which precipitates cytoplasmic proteins and inactivates enzyme systems within fungal cell. Glycerol prevents drying, allowing for examination of the stain 18-24 hours after preparation. Lactic acid is a clearing agent. Aniline blue is a dye which stains the hyaline fungal structures making them more distinct.

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