SiteClick™ Qdot™ Antibody Labeling Kits
SiteClick™ Qdot™ Antibody Labeling Kits
Invitrogen™

SiteClick™ Qdot™ Antibody Labeling Kits

Achieve precise and reproducible antibody labeling with SiteClick R-PE Antibody Labeling Kit.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberLabel or Dye
S10453Qdot 655
S10449Qdot 525
S10454Qdot 705
S10450Qdot 565
S10451Qdot 585
S10452Qdot 625
S10455Qdot 800
S10469Qdot 605
Catalog number S10453
Price (CNY)
7,944.00
Each
Add to cart
Label or Dye:
Qdot 655
Price (CNY)
7,944.00
Each
Add to cart

Achieve precise and reproducible antibody labeling with SiteClick R-PE Antibody Labeling Kit. This kit targets carbohydrate domains present on all IgG antibodies, regardless of isotype or host species, ensuring site-selective labeling. The labeled antibodies are suitable for flow cytometry, fluorescence imaging, and western blot detection.

Features of the SiteClick R-PE Antibody Labeling Kit include:

  • Complete kit—includes everything needed to label 100 μg of IgG antibody using a straightforward, step-by-step protocol
  • Site-specific labeling—SiteClick technology attaches labels to the heavy chains of IgG antibodies under mild conditions, preserving antigen-binding domains
  • Consistent results—the gentle, site-selective attachment to heavy-chain N-linked glycans ensures reproducibility across sequential labelings and different antibodies, without harsh reduction steps
  • Efficient protocol—easy-to-follow steps requiring minimal hands-on time
  • High-quality conjugates—produces highly efficient, site-specific, and reproducible antibody conjugates

Unlike traditional amine or thiol labeling, which can affect antigen binding domains or antibody structure, SiteClick labeling technology maintains the integrity of the antibody. This ensures that antigen binding sites remain available for target binding, providing consistent and reproducible labeling. The workflow utilizes copper-free click chemistry to covalently link the DIBO moiety with the azide-modified antibody, ensuring uniform labeling without the need for reaction optimization.

With the SiteClick R-PE Antibody Labeling Kit, you can confidently achieve consistent, high-quality labeling every time.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorFar-Red
Excitation/Emission405/655
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Product LineQdot™, SiteClick™
Product TypeLabeling Kit
Quantity1 kit
Shipping ConditionWet Ice
Labeling TargetIgG
Label or DyeQdot 655
Unit SizeEach
Contents & Storage
Store the entire kit at 2 to 6°C upon receipt

Frequently asked questions (FAQs)

I need to label my tissue section with two mouse primary antibodies. Do I have to make direct conjugates of the antibodies to use them?

Check the isotype of the two antibodies to see if they are different. If so, we offer isotype-specific goat anti-mouse secondary antibodies that can be used together with minimal cross-reactivity. If the two mouse antibodies are of the same isotype, then you would need to make direct conjugates, such as with our antibody labeling kits or APEX, and SiteClick kits.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.