SiteClick™ Biotin Antibody Labeling Kit, 1 kit

Achieve precise and reproducible antibody labeling with SiteClick Biotin Antibody Labeling Kits. This kit targets carbohydrate domains present on all IgG antibodies, regardless of isotype or host species, ensuring site-selective labeling. The labeled antibodies are suitable for flow cytometry, fluorescence imaging, and western blot detection.

Features of the SiteClick Biotin Antibody Labeling Kit include:

• Complete kit—includes everything needed to label 100–250 μg of IgG antibody using a straightforward, step-by-step protocol
• Site-specific labeling—SiteClick technology attaches labels to the heavy chains of IgG antibodies under mild conditions, preserving antigen binding domains
• Consistent results—the gentle, site-selective attachment to heavy-chain N-linked glycans ensures reproducibility across sequential labelings and different antibodies, without harsh reduction steps

• Efficient protocol—easy-to-follow steps requiring minimal hands-on time
• High-quality conjugates—produces highly efficient, site-specific, and reproducible antibody conjugates

Unlike traditional amine or thiol labeling, which can affect antigen binding domains or antibody structure, SiteClick labeling technology maintains the integrity of the antibody. This ensures that antigen binding sites remain available for target binding, providing consistent and reproducible labeling. The workflow utilizes copper-free click chemistry to covalently link the DIBO moiety with the azide-modified antibody, ensuring uniform labeling without the need for reaction optimization.

With the SiteClick Biotin Antibody Labeling Kit, you can confidently achieve consistent, high-quality labeling every time.