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Citations & References (7)
Invitrogen™
SNARF™-4F 5-(and-6)-Carboxylic Acid
The cell-impermeant ratiometric pH indicator SNARF™-4F 5-(and-6)-carboxylic acid (pKa of ∼6.4) exhibits a significant pH-dependent emission shift from yellow-orange toRead more
| Catalog Number | Quantity |
|---|---|
| S23920 | 1 mg |
Catalog number S23920
Price (CNY)
4,815.00
Online Exclusive
Ends: 31-Dec-2025
6,316.00Save 1,501.00 (24%)
Each
Quantity:
1 mg
Price (CNY)
4,815.00
Online Exclusive
Ends: 31-Dec-2025
6,316.00Save 1,501.00 (24%)
Each
The cell-impermeant ratiometric pH indicator SNARF™-4F 5-(and-6)-carboxylic acid (pKa of ∼6.4) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeFluorescent Dye-Based
IndicatorpH Indicator
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineSNARF
Product TypepH Indicator
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Citations & References (7)
Citations & References
Abstract
Application of a pH-sensitive fluoroprobe (C-SNARF-4) for pH microenvironment analysis in Pseudomonas aeruginosa biofilms.
Journal:Appl Environ Microbiol
PubMed ID:15870340
'An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorhodafluor-4F 5-(and-6) carboxylic acid (C-SNARF-4)
Characterization of dual-wavelength seminaphthofluorescein and seminapthorhodafluor dyes for pH sensing under high hydrostatic pressures.
Journal:Anal Biochem
PubMed ID:17274941
'Hydrostatic pressure is an important physical parameter in biology, with pressures in the few-hundred-atm range having significant effects on cellular morphology, metabolism, and viability. To ensure valid results when studying pressure effects using fluorescence spectroscopy and imaging methods, metabolic probes need to be characterized for high-pressure use. Of interest is
Fluorescent indicators for intracellular pH.
Journal:Chem Rev
PubMed ID:19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980.
Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.
Journal:Bioorg Med Chem Lett
PubMed ID:11677123
Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission
Single cell kinetics of intracellular, nonviral, nucleic acid delivery vehicle acidification and trafficking.
Journal:Bioconjug Chem
PubMed ID:16029041
Mechanistic understanding of the intracellular trafficking of nonviral nucleic acid delivery vehicles remains elusive. A live, single cell-based assay is described here that is used to investigate and quantitate the spatiotemporal, intracellular pH microenvironment of polymeric-based nucleic acid delivery vehicles. Polycations such as polyethylenimine (PEI), poly-l-lysine (PLL), beta-cyclodextrin-containing polymers lacking