SNARF™-5F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging
SNARF™-5F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging
Citations & References (13)
Invitrogen™

SNARF™-5F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging

The cell-permeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid, acetoxymethyl ester, acetate (pKa of ∼7.2) exhibits a significant pH-dependent emission shiftRead more
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Catalog NumberQuantity
S2392320 x 50 μg
Catalog number S23923
Price (CNY)
6,184.00
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Ends: 31-Dec-2026
8,471.00
Save 2,287.00 (27%)
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Quantity:
20 x 50 μg
Price (CNY)
6,184.00
飞享价
Ends: 31-Dec-2026
8,471.00
Save 2,287.00 (27%)
Each
Add to cart
The cell-permeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid, acetoxymethyl ester, acetate (pKa of ∼7.2) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeFluorescent Dye-Based
IndicatorpH Indicator
Quantity20 x 50 μg
Shipping ConditionRoom Temperature
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineSNARF
Product TypepH Indicator
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (13)

Citations & References
Abstract
Relationships between calcium and pH in the regulation of the slow afterhyperpolarization in cultured rat hippocampal neurons.
Authors:Kelly T, Church J
Journal:J Neurophysiol
PubMed ID:16885515
'The Ca(2+)-dependent slow afterhyperpolarization (AHP) is an important determinant of neuronal excitability. Although it is established that modest changes in extracellular pH (pH(o)) modulate the slow AHP, the relative contributions of changes in the priming Ca(2+) signal and intracellular pH (pH(i)) to this effect remain poorly defined. To gain a ... More
Potential contribution of a voltage-activated proton conductance to acid extrusion from rat hippocampal neurons.
Authors:Cheng YM, Kelly T, Church J,
Journal:Neuroscience
PubMed ID:18201832
'We examined the potential contribution of a voltage-gated proton conductance (gH+) to acid extrusion from cultured postnatal rat hippocampal neurons. In neurons loaded with Ca2+- and/or pH-sensitive fluorophores, transient exposures to 25-139.5 mM external K+ (K+o) or 20 microM veratridine in the presence of 2 mM Ca2+o (extracellular pH (pHo) ... More
Fluorescent indicators for intracellular pH.
Authors:Han J, Burgess K,
Journal:Chem Rev
PubMed ID:19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.
Authors:Liu J, Diwu Z, Leung WY
Journal:Bioorg Med Chem Lett
PubMed ID:11677123
Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission ... More
Time-domain fluorescence lifetime imaging for intracellular pH sensing in living tissues.
Authors:Hille C, Berg M, Bressel L, Munzke D, Primus P, Löhmannsröben HG, Dosche C,
Journal:Anal Bioanal Chem
PubMed ID:18481048
pH sensing in living cells represents one of the most prominent topics in biochemistry and physiology. In this study we performed one-photon and two-photon time-domain fluorescence lifetime imaging with a laser-scanning microscope using the time-correlated single-photon counting technique for imaging intracellular pH levels. The suitability of different commercial fluorescence dyes ... More
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