Streptavidin, Alexa Fluor™ 405 conjugate
Streptavidin, Alexa Fluor™ 405 conjugate
Citations & References (4)
Invitrogen™

Streptavidin, Alexa Fluor™ 405 conjugate

Our streptavidin, Alexa Fluor 405 conjugate is a bright, photostable probe with excitation/emission maxima of 402 and 421 nm. StreptavidinRead more
Have Questions?
Catalog NumberQuantity
S323511 mg
Catalog number S32351
Price (CNY)
4,723.00
Each
Add to cart
Quantity:
1 mg
Price (CNY)
4,723.00
Each
Add to cart
Our streptavidin, Alexa Fluor 405 conjugate is a bright, photostable probe with excitation/emission maxima of 402 and 421 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission402/421
Flow Cytometer Laser Lines405
For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Label or DyeAlexa Fluor 405
Product TypeStreptavidin Conjugate (Fluorescent)
Quantity1 mg
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 405
FormSolid
Product LineAlexa Fluor
Unit SizeEach
Contents & Storage
Contains 1 vial of streptavidin Alexa Fluor™ 405 conjugate.

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (4)

Citations & References
Abstract
Violet laser diodes in flow cytometry: an update.
Authors:Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R,
Journal:Cytometry A
PubMed ID:17051581
INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More
Novel strategy to selectively label excitatory and inhibitory neurons in the cerebral cortex of mice.
Authors:Basu K, Gravel C, Tomioka R, Kaneko T, Tamamaki N, Sík A,
Journal:J Neurosci Methods
PubMed ID:18321591
Revealing the connections of neuronal systems is critical for understanding how they function. The vast majority of neurons in all cortical areas consist of excitatory cells whose activity is controlled by inhibitory cells. Distribution and projection patterns of inhibitory and excitatory cells are key information to understand the organization of ... More
Endosomal trafficking of the ligated FcvarepsilonRI receptor.
Authors:Fattakhova GV, Masilamani M, Narayanan S, Borrego F, Gilfillan AM, Metcalfe DD, Coligan JE,
Journal:Mol Immunol
PubMed ID:18945491
In addition to initiating signaling cascades leading to mast cell mediator release, aggregation of the high affinity IgE receptor (FcvarepsilonRI) leads to rapid internalization of the cross-linked receptor. However, little is known about the trafficking of the internalized FcvarepsilonRI. Here we demonstrate that in RBL-2H3 cells, aggregated FcvarepsilonRI appears in ... More
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.
Authors:Pitulescu ME, Schmidt I, Benedito R, Adams RH,
Journal:Nat Protoc
PubMed ID:20725067
The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The ... More