Streptavidin, Allophycocyanin Conjugate, premium grade

Citations & References (88)

Invitrogen™

Streptavidin, Allophycocyanin Conjugate, premium grade

Catalog Number	Quantity
S32362 also known as S-32362	250 μL

Catalog number S32362

also known as S-32362

Price (CNY)

12,472.00

Quantity:

250 μL

Price (CNY)

12,472.00

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than does avidin. Allophycocyanin is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ∼650 nm, and a fluorescence emission maximum of ∼660 nm. Our streptavidin allophycocyanin conjugate *premium grade* is a further fractionation of our S-868 product, intended especially for applications that benefit from additional purity.

Related Links:

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration1 mg/mL

Detection MethodFluorescence

Excitation/Emission650/660

Flow Cytometer Laser Lines633 or 635

For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis

Label or DyeAPC (Allophycocyanin)

Product TypeStreptavidin Conjugate (Fluorescent)

Quantity250 μL

Shipping ConditionWet Ice

ConjugateAPC

FormSolution

Unit SizeEach

Contents & Storage

Contains 1 vial of streptavidin APC, premium grade, conjugate (1 mg/mL).

Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

For Streptavidin, Allophycocyanin Conjugate, premium grade (Cat. No. S32362), how many molecules of APC are conjugated to each streptavidin molecule?

Streptavidin APC, premium grade has a degree of labeling of one APC molecule to one streptavidin molecule.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store my allophycocyanin (APC)-conjugated antibody?

Allophycocyanin-conjugated antibodies must be stored at 2-8 degrees C. Antibodies conjugated to phycobiliproteins (APC, R-PE) must never be frozen. Once frozen, even briefly, they are no longer usable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (88)

Citations & References

Abstract

Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.

Authors:Chattopadhyay PK, Melenhorst JJ, Ladell K, Gostick E, Scheinberg P, Barrett AJ, Wooldridge L, Roederer M, Sewell AK, Price DA,

Journal:Cytometry A

PubMed ID:18836993

'The ability to quantify and characterize antigen-specific CD8+ T cells irrespective of functional readouts using fluorochrome-conjugated peptide-major histocompatibility complex class I (pMHCI) tetramers in conjunction with flow cytometry has transformed our understanding of cellular immune responses over the past decade. In the case of prevalent CD8+ T cell populations that ... More

Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.

Authors:Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP

Journal:Anal Biochem

PubMed ID:12419349

'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More

Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts.

Authors:Gray A, Olsson H, Batty IH, Priganica L, Peter Downes C

Journal:Anal Biochem

PubMed ID:12605860

'We describe a novel approach to quantitation of phosphoinositides in cell extracts and in vitro enzyme-catalyzed reactions using suitably tagged and/or labeled pleckstrin homology (PH) domains as probes. Stable complexes were formed between the biotinylated target lipid and an appropriate PH domain, and phosphoinositides present in samples were detected by ... More

Development of a ubiquitin transfer assay for high throughput screening by fluorescence resonance energy transfer.

Authors:Boisclair MD, McClure C, Josiah S, Glass S, Bottomley S, Kamerkar S, Hemmilä I

Journal:J Biomol Screen

PubMed ID:11080690

'An assay based on fluorescence resonance energy transfer (FRET) has been developed to screen for ubiquitination inhibitors. The assay measures the transfer of ubiquitin from Ubc4 to HECT protein Rsc 1083. Secondary reagents (streptavidin and antibody to glutathione-S-transferase [GST]), pre-labeled with fluorophores (europium chelate, Eu(3+), and allophycocyanin [APC]), are noncovalently ... More

Cell surface markers in human embryonic stem cells.

Authors:Rao RR, Johnson AV, Stice SL,

Journal:Methods Mol Biol

PubMed ID:18453248

'The pluripotent nature of human embryonic stem cells (hESCs) is based on their potential to form every cell type in the body. Prior to use in directed differentiation strategies, these cells need to be thoroughly characterized. The large number of glycoproteins and carbohydrates that exist on the cell surface provide ... More

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