Streptavidin Agarose
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Invitrogen™

Streptavidin Agarose

Streptavidin Agarose is suitable for isolating biotinylated molecules and complexes with biotinylated components, including antibodies, antibody-antigen complexes, ligands and receptors,Read more
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Catalog NumberQuantity
SA10004
also known as SA100-04
5 mL
Catalog number SA10004
also known as SA100-04
Price (CNY)
7,354.00
Each
Add to cart
Quantity:
5 mL
Request bulk or custom format
Price (CNY)
7,354.00
Each
Add to cart
Streptavidin Agarose is suitable for isolating biotinylated molecules and complexes with biotinylated components, including antibodies, antibody-antigen complexes, ligands and receptors, nucleic acids, and nucleic acid-protein complexes. It is especially useful as an immunoprecipitation reagent for antibodies that do not bind well to protein A. The matrix consists of streptavidin covalently attached to cross-linked 4% agarose beads through a 15-atom spacer arm. Streptavidin Agarose can bind 3 to 8 mg of biotinylated IgG/ml of gel.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10 mg/mL
Product TypeStreptavidin Agarose
Quantity5 mL
FormLiquid Suspension
Unit SizeEach
Contents & Storage
Streptavidin Agarose is supplied as 10 ml of a 50% slurry containing 5 ml of packed gel bed in 0.01 M sodium phosphate (pH 7.2), 0.15 M NaCl, and 0.02% sodium azide. Store at 4°C.

Frequently asked questions (FAQs)

How many biotin binding-sites are there per streptavidin molecule?

Streptavidin is a protein composed of four identical subunits, each containing a high affinity binding site for biotin (K-D = 10 -15 M) . Streptavidin has the same biotin binding properties as avidin, but it has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low non-specific binding.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the binding capacity of streptavidin agarose?

The binding capacity of streptavidin agarose is 15-20 µg (18-24 nmoles) of fluorescein biotin per mL of sedimented gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.