iBind™ Flex Cards

iBind Flex Cards are used with the iBind Flex Western Device, which is an automated western-processing device that performs everyRead more

Catalog Number	Type	Quantity
SLF2010	Card	10 Cards

Catalog number SLF2010

Price (CNY)

3,697.00

10 cards

Add to cart

Type:

Quantity:

10 Cards

Price (CNY)

3,697.00

10 cards

Add to cart

iBind Flex Cards are used with the iBind Flex Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. Each solution is released from the iBind Flex device wells to the iBind Flex Card through sequential lateral flow (SLF); the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Learn more about the iBind western systems ›

The iBind Flex Cards, together with the iBind Flex Western Device and iBind Flex Solution Kit or iBind Flex Fluorescent Detection (FD) Solution Kit, bring automation and simplicity to your western protocol. Each iBind Flex Card can process up to one midi blot, two mini blots, or six vertically-cut strip blots.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity10 Cards

Shipping ConditionRoom Temperature

Capacity1 Midi Blot, 6 Vertically-Cut Strip Blots, 2 Mini Blots

Detection MethodChromogenic, Chemiluminescence, Fluorescence

For Use With (Application)Western Blot

For Use With (Equipment)iBind Flex Western Device

Membrane CompatibilityNitrocellulose, PVDF

Product LineiBind Flex

Running Time∼2.5 hr.

Throughput1 Midi Blot, 6 Vertically-Cut Strip Blots, 2 Mini Blots/Card

TypeCard

Unit Size10 cards

Frequently asked questions (FAQs)

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBind™ Flex Cards, 10 Cards FAQs