Staphylococcal Enterotoxin Reversed Passive Latex Agglutination Kit (SET- RPLA)

Rapidly detect staphylococcal enterotoxins A, B, C and D in food samples or culture filtrates with Thermo Scientific™ Oxoid™ Staphylococcal Enterotoxin Reversed Passive Latex Agglutination Kit (SET-RPLA). The technique of reversed passive latex agglutination (RPLA) enables soluble antigens such as bacterial toxins to be detected in an agglutination assay.

The SET-RPLA can be used to detect staphylococcal enterotoxins in a wide variety of foods and to give a semi-quantitative result.

Staphylococcal food poisoning is caused by eating foods contaminated with enterotoxins produced during the growth of certain strains of Staphylococcus aureus. The enterotoxins A, B, C, D and E play an important role in the pathogenesis of staphylococcal food poisoning which is normally characterized by nausea, vomiting and diarrhoea.

Use Staphylococcal Enterotoxin RPLA test for simple and semi-quantitative detection of staphylococcal enterotoxins in food samples.

• Simple – easy to implement
• Visual – easy to read results

In a standard agglutination assay, soluble antibody reacts with particulate antigen such as bacterial cells. However, in a REVERSED agglutination assay the antibody, which is attached to particles, reacts with the soluble antigen. The particles (in this case, latex) do not themselves play a part in the reaction and they are therefore PASSIVE. The cross-linking of the latex particles by the specific antigen/antibody reaction results in the visible LATEX AGGLUTINATION reaction.

The SET-RPLA test kit is based upon the reports by Shingaki et al.¹ and Oda et al.² It was developed under the guidance of the Tokyo Metropolitan Research Laboratory of Public Health.

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