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Thermo Scientific™
Pierce™ p-Bromophenacylate Reagent
Label, detect and measure peptides and amino acids by HPLC using this carboxylic acid-reactive compound (Amax 260nm; UV); also called p-Bromophenacyl-8.
Catalog number TS-48891
Price (CNY)
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Thermo Scientific™ Pierce p-Bromophenacylate Reagent reacts with carboxylic acids to provide quantitative detection of peptides and amino acids with few or no side reactions.
Highlights:
- Premixing of phenacylbromide and crown ether is not necessary
- Derivatization is both rapid and quantitative, with yields of >95% in 15 to 20 minutes at 80°C
- Excess reactants do not interfere
- Large excess of alkylating reagent is not necessary
- Small amounts of water or alcohol do not interfere
- If isolation is desired, products are usually crystalline
Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
General References:
1. Durst, H.D., et al. (1975). Anal. Chem. 47, 1797.
2. Borch, R.F., et al. 1975). Anal. Chem. 47, 2437.
3. Grushka, E., et al. (1975). J. Chromatogr. 112, 673.
4. Fitzpatrick, F.A., et al. (1976). Anal. Chem. 48, 499.
Specifications
DescriptionPierce p-Bromophenacylate Reagent
FormulationSolution of 0.1mmol/mL p-bromophenacyl bromide and 0.005mmol/mL crown ether in acetonitrile
Product TypeReagent
Standard Concentration0.1, 0.005 mmole/mL
Sufficient ForDerivatizing Approx. 10 x 10 mg Amino Acid Samples Using Typical Procedure
Detection MethodUV Absorption (260 nm)
FormatLiquid
Quantity10 mL
For Use With (Application)HPLC
Unit SizeEach