pAd/BLOCK-iT™-DEST RNAi Gateway Vector
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Citations & References (2)
Invitrogen™

pAd/BLOCK-iT™-DEST RNAi Gateway Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,Read more
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Catalog NumberQuantity
V49220
also known as V492-20
6 μg
Catalog number V49220
also known as V492-20
Price (CNY)
18,873.00
6 µg
Add to cart
Quantity:
6 μg
Price (CNY)
18,873.00
6 µg
Add to cart
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeAdenoviral
Product TypeRNAi Gateway Vector
Quantity6 μg
VectorpAd, pDEST
Cloning MethodGateway
Product LineBLOCK-iT, Gateway
PromoterU6
Unit Size6 µg
Contents & Storage
All destination vectors are provided lyophilized and supercoiled.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pAd/BLOCK-iT™-DEST RNAi Gateway Vector FAQs

Citations & References (2)

Citations & References
Abstract
Thymidine phosphorylase is angiogenic and promotes tumor growth.
Authors:Moghaddam A, Zhang HT, Fan TP, Hu DE, Lees VC, Turley H, Fox SB, Gatter KC, Harris AL, Bicknell R
Journal:Proc Natl Acad Sci U S A
PubMed ID:7532308
'Platelet-derived endothelial cell growth factor was previously identified as the sole angiogenic activity present in platelets; it is now known to be thymidine phosphorylase (TP). The effect of TP on [methyl-3H]thymidine uptake does not arise from de novo DNA synthesis and the molecule is not a growth factor. Despite this, ... More
Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo.
Authors:Gou D, Narasaraju T, Chintagari NR, Jin N, Wang P, Liu L,
Journal:Nucleic Acids Res
PubMed ID:15452203
RNA interference (RNAi) is a sequence-specific post-transcriptional gene silencing process. Although it is widely used in the loss-of-function studies, none of the current RNAi technologies can achieve cell-specific gene silencing. The lack of cell specificity limits its usage in vivo. Here, we report a cell-specific RNAi system using an alveolar ... More