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Thermo Scientific™
Varioskan™ LUX multimode microplate reader, Absorbance, Fluorescence intensity and Luminescence
Analyze a variety of samples with five measurement technologies, including absorbance (UV-Vis), fluorescence intensity, Luminescence, AlphaScreen / AlphaLISA and TRF.
Catalog number VL0L00D2
Price (CNY)Request A Quote
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Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific™ Varioskan™ LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. The instrument simplifies measurement setup with the automatic dynamic range selection, and its smart safety controls ease workflow and help avoid experimental errors. Varioskan LUX raises the bar for reliability and ease, with technology that works in researchers' best interest.
The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.
The Varioskan LUX is a modular, upgradable instrument that provides excellent usability across a variety of applications.
- Up to five measurement technologies: Absorbance (UV-Vis), Fluorescence intensity (incl. FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA and Time-resolved fluorescence (incl. TR-FRET/hTRF)
- Filter and monochromator optics
- Spectral scanning for assay optimization
- Five measurement modes including end point, kinetic, spectra, kinetic spectra and multipoint
- Integrated gas control module for setting CO2 and O2 concentrations for cell based assays
- Simultaneous dispensing and measurement for signal monitoring at the start of a reaction
- Automatic dynamic range selection, based on signal intensity of each well
- Smart safety controls designed to identify and alert the user of potential problems before they happen
- Automated instrument calibration and self-diagnostics for reliable performance
- Certified HTRF compatible reader by CisBio Bioassays
- Validated DLReady by Promega Corporation
Varioskan LUX is paired with the powerful and intuitive SkanIt Software, providing excellent usability and flexibility.
- License free—Each user can install the software on his/her computer
- Intuitive and flexible interface for multiple users and assays
- Innovative Virtual Pipetting Tool makes it easy to define samples to plate layout
- Measurement data is continuously saved to the database, so there's no data lost due to unexpected interruptions such as power outages or accidental aborting
- Simplified data processing and export
- Export data in a single mouse click, or have it emailed automatically at the end of the run
- Several file formats for exporting results: *.xlsx, *.pdf, *.xml, and *.txt
- Shows real-time spectra and kinetic curves during the measurement
- Built-in calculations for data analysis (e.g. blank subtraction, average/SD/CV%, standard curve, dose response, quality control, classification, kinetic and spectral analysis, custom formula, etc.)
- Comprehensive result reports with traceability to runtime actions and settings
- Two editions available: Research Edition for life science researchers, and Drug Discovery Edition which offers features needed for the FDA 21 CFR Part 11 compliance.
The Varioskan LUX is modular and upgradeable, so you can build the system that best suits your needs. For more information, contact a sales representative.
Specifications
Accuracy0.003 Abs or ±2%, at 200 to 399 nm (0 to 2 Abs)
0.003 Abs or ±1%, at 400 to 1000 nm (0 to 3 Abs)
0.003 Abs or ±1%, at 400 to 1000 nm (0 to 3 Abs)
CompatibilityRobot
Depth (English)23 in.
Depth (Metric)58 cm
Dispense Volume1 mL syringe: 2 to 5,000 μL
5 mL syringe: 5 to 25,000 μL
5 mL syringe: 5 to 25,000 μL
Dynamic RangeFluorescence intensity: top reading > 6 decades
Luminescence: > 7 decades
Luminescence: > 7 decades
Height (English)20 in.
Height (Metric)51 cm
Linearity96-well plate: ±2% (0 to 4 Abs at 450 nm)
384-well plate: ±2% (0 to 3 Abs at 450 nm)
384-well plate: ±2% (0 to 3 Abs at 450 nm)
Measurement Speed15 s for 96-well Plate, 45 s for 384-well Plate, 135 s for 1536-Well Plate (Minimum Times)
Measurement TypeEndpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra
No. of Dispensers2
PrecisionSD < 0.001 Abs or CV < 0.5%, at 450 nm (0 to 3 Abs)
SensitivityFluorescence intensity (top reading): < 0.4 fmol fluorescein/well (black 384-well plate)
Luminescence: < 7 amol ATP/well (white 384-well plate)
Luminescence: < 7 amol ATP/well (white 384-well plate)
Software Language VersionsEnglish, German, French, Spanish, Portuguese, Italian, Japansese, Chinese and Russian
Spectral Scanning Speed<2.2 s/Well 400 to 500 nm, 1 Flash, 2 nm Step
Wavelength RangeAbsorbance: 200 to 1000 nm
Fluorescence intensity: Excitation: 200 to 1000 nm, Emission: 270 to 840 nm
Luminescence: 360 to 670 nm
Fluorescence intensity: Excitation: 200 to 1000 nm, Emission: 270 to 840 nm
Luminescence: 360 to 670 nm
Weight (English)128 lb.
Weight (Metric)58 kg
Width (English)21 in.
Width (Metric)53 cm
Detector TypePhotomultiplier Tube (PMT)
Incubator Temperature45°C
InterfacePC Software (Thermo Scientific SkanIt Software)
Light SourceXenon Flash Lamp
Measurement TechnologyAbsorbance, Fluorescence Intensity, Luminescence
Plate Type6 to 1536-well Plates (Absorbance 6 to 384-well Plates)
Product LineVarioskan
Reading OptionsTop
Read-out Range0 to 6 Abs
ShakingOrbital
Software TypeSkanIt Software
Wavelength SelectionAbsorbance & Spectral Scanning: Double Monochromator, Fluorescence Intensity: Double Excitation and Emission Monochromator
Unit SizeEach