Novex™ HRP Chromogenic Substrate (TMB)
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Invitrogen™

Novex™ HRP Chromogenic Substrate (TMB)

Novex® HRP Chromogenic Substrate is a ready to use single component reagent used for sensitive detection of a horse radishRead more
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Catalog NumberQuantity
WP200041 x 250 ml bottle
Catalog number WP20004
Price (CNY)
3,850.00
250 mL
Add to cart
Quantity:
1 x 250 ml bottle
Price (CNY)
3,850.00
250 mL
Add to cart
Novex® HRP Chromogenic Substrate is a ready to use single component reagent used for sensitive detection of a horse radish peroxidase (HRP) on western blot and dot blot membranes. Consisting of a ready-to-use solution of TMB, the Novex® HRP Chromogenic Substrate forms a blue precipatate up reaction with horse radish peroxidase (HRP).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
Quantity1 x 250 ml bottle
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Substrate PropertiesChemical Substrate
Substrate TypeHRP (Horseradish Peroxidase) Substrate
Detection MethodColorimetric
FormLiquid
Product LineNovex
SubstrateTMB
Unit Size250 mL
Contents & Storage
Single bottle labelled: Novex™ HRP Chromogenic Substrate (TMB). Avoid exposure to light. Store at 4°C.

Frequently asked questions (FAQs)

What are the Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB)?

- Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB) are ready-to-use, single component substrates for sensitive immunodetection of alkaline phosphatase (AP) or horse radish peroxidase (HRP), respectively, on western blots or dot blots. The sensitivity of the AP substrate is in the 100 pg range, while the HRP substrate is in the 1 ng range (see Note below).

- Invitrogen AP Chromogenic Substrate consists of a ready-to-use solution of 5-bromo-4-chloro-3-indolyl-1-phosphate (BCIP) and nitroblue tetrazolium (NBT) which forms a black-purple precipitate upon reaction with alkaline phosphatase. It is available as a standalone product (Cat. Nos. WP20001) and is also a component of the WesternBreeze Chromogenic Detection Kits.

- Invitrogen HRP Chromogenic Substrate consists of a ready-to-use non-toxic solution of 3,3',5,5'-tetramethylbenzidine (TMB) which forms a blue precipitate upon reaction with horse radish peroxidase. It is available as a standalone product (Cat. No. WP20004).

Note: Sensitivity may vary depending upon the antigen and antibody used for detection.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.