Which probe array cartridge carriers are designed specifically for use with the GeneChip Hybridization Oven 645 (Cat. No. 00-0331)?
The following probe array cartridge carriers are designed for use with the GeneChip Hybridization Oven 645:
- Probe Array Cartridge Carriers (Red) (Cat. No. 90-0356)
- Probe Array Cartridge Carriers (Green) (Cat. No. 90-0357)
- Probe Array Cartridge Carriers (Purple) (Cat. No. 90-0358)
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Why is it important to use Tough-Spots® labels when using GeneChip cartridge arrays?
Tough-Spots® labels are small adhesive stickers used to temporarily seal the backs of cartridge arrays during the overnight hybridization step. They are required to prevent loss of volume due to evaporation through the septa. We recommend using Tough-Spots® labels on Rolls from USA Scientific (Item No. 9185-0000)
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What are the respective hybridization volumes based on array format for Affymetrix gene expression arrays?
Proper hybridization volume is critical to obtaining an even signal across a given array. Too little volume can lead to black circles in the middle of the array. Too much volume can leak out of the back of the array. The correct hybridization volume leaves enough room for a small air bubble to circulate around the array surface during the overnight hybridization. Here are the recommended hybridization and fill volumes based on the array format:
Array Format; Hybridization Volume; Fill Volume
- 49 Format (Standard); 200 µL; 250 µL
- 64 Format; 200 µL; 250 µL
- 100 Format (Midi); 130 µL; 160 µL
- 169 Format (Mini); 80 µL; 100 µL
- 400 Format (Micro); 80 µL; 100µL
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What components are needed to run the Affymetrix Mouse Diversity Genotyping Array?
The components include: Affymetrix Mouse Diversity Genotyping Array
Affymetrix Genome-Wide Human SNP Nsp/Sty Assay kit 5.0/6.0
Affymetrix Power Tools (sed to run a modified BRLMM-P algorithm for genotype calling, GeneChip Fluidics Station 450
GeneChip Hybridization Oven 645
and GeneChip Scanner 3000 7G.
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What is the minimum order size for the Mouse Diversity Genoytping array?
Customers must purchase a minimum of 60 arrays to begin a study, because this number of samples is required for BLRMM-P to cluster the data properly. For subsequent orders, the minimum order is approximately 30 arrays.
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