膜封闭溶液

货号	数量
000105	1 L

货号 000105

价格（CNY)

2,547.00

价格（CNY)

2,547.00

膜封闭溶液可用于封闭硝酸纤维素膜和 PVDF 膜以及稀释的抗体或酶偶联物。该溶液是一种含有牛血清白蛋白、山羊 IgG、Tween 20 和可以降低非特异性结合的其他组分的混合物的 Tris 缓冲液。该试剂中使用的 Proclin 不会抑制过氧化物酶或碱性磷酸酶活性。请注意，该封闭溶液含有正常的山羊 IgG，不得用于使用抗山羊免疫球蛋白的检测。

仅供科研使用。不可用于诊断程序。

化学名称或材料膜封闭溶液

建议的储存条件该试剂在 2-8°C 下储存。切勿冷冻。如果发生沉淀，则在使用前进行过滤以澄清。

数量1 L

Unit SizeEach

常见问题解答 (FAQ)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

引用和文献 (1)

引用和文献

Abstract

Serine phosphorylation of p60 tumor necrosis factor receptor by PKC-delta in TNF-alpha-activated neutrophils.

Authors:Kilpatrick LE, Song YH, Rossi MW, Korchak HM,

Journal:Am J Physiol Cell Physiol

PubMed ID:11078718

Tumor necrosis factor-alpha (TNF-alpha) triggers degranulation and oxygen radical release in adherent neutrophils. The p60TNF receptor (p60TNFR) is responsible for proinflammatory signaling, and protein kinase C (PKC) is a candidate for the regulation of p60TNFR. Both TNF-alpha and the PKC-activator phorbol 12-myristate 13-acetate triggered phosphorylation of p60TNFR. Receptor phosphorylation was ... More