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引用和文献 (4)
Invitrogen™
重组蛋白 G - 琼脂糖™ 4B
重组蛋白 G 在一种大肠杆菌菌株中生产，该菌株含有链球菌属的蛋白 G 基因。本产品通过将高纯度重组蛋白 G 共价偶联至 Sepharose™ 4B了解更多信息
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| 货号 | 数量 |
|---|---|
| 101241 | 2 mL |
| 101242 | 5 mL |
| 101243 | 25 mL |
货号 101241
价格（CNY)
4,208.00
Each
数量:
2 mL
价格（CNY)
4,208.00
Each
重组蛋白 G 在一种大肠杆菌菌株中生产，该菌株含有链球菌属的蛋白 G 基因。本产品通过将高纯度重组蛋白 G 共价偶联至 Sepharose™ 4B 制备而成。本产品以 50% 悬浮液的形式提供，包含 25 mL 重组蛋白 G-Sepharose™ 4B + 25 mL PBS（10 mM 磷酸盐缓冲盐水，pH 值7.4，含 0.19% Kathon CG/ICP）。
应用：免疫球蛋白亲和纯化和免疫沉淀。
重组蛋白 G 与许多类型的免疫球蛋白 G 的 Fc 区结合，包括：
• 所有四种亚类的人 IgG
• 小鼠 IgG
• 兔 IgG
• 豚鼠 IgG
• 大鼠 IgG
• 山羊 IgG
• 绵羊 IgG
• 牛 IgG
• 马 IgG
重组蛋白 G 不与鸡或猫 IgG 或者人 IgA、IgM 或血清白蛋白结合。它与犬 IgG 发生较弱结合。
应用：免疫球蛋白亲和纯化和免疫沉淀。
重组蛋白 G 与许多类型的免疫球蛋白 G 的 Fc 区结合，包括：
• 所有四种亚类的人 IgG
• 小鼠 IgG
• 兔 IgG
• 豚鼠 IgG
• 大鼠 IgG
• 山羊 IgG
• 绵羊 IgG
• 牛 IgG
• 马 IgG
重组蛋白 G 不与鸡或猫 IgG 或者人 IgA、IgM 或血清白蛋白结合。它与犬 IgG 发生较弱结合。
仅供科研使用。不可用于诊断程序。
规格
形式悬浮
数量2 mL
色谱柱类型亲和
产品线琼脂糖
产品类型蛋白 G
固定相蛋白 G
Unit SizeEach
内容与储存
本产品以 50% 悬浮液的形式提供（2 mL 重组蛋白 G-Sepharose™ 4B + 2 mL PBS）湿冰
常见问题解答 (FAQ)
Can I perform a centrifugation separation with Recombinant Protein G - Sepharose 4B (Cat. Nos. 101241, 101242, 101243) instead of column separation?
引用和文献 (4)
引用和文献
Abstract
Inducible T cell antigen 4-1BB. Analysis of expression and function.
Journal:J Immunol
PubMed ID:7678621
'4-1BB is an inducible receptor-like protein expressed in both cytolytic and Th cells. Optimal induction of 4-1BB mRNA in T cells required both PMA and ionomycin stimulation, indicating that protein kinase C activation and increases in intracellular Ca2+ were required for its expression. 4-1BB was categorized as an early activation
The glycosylation of IgA produced by murine B cells is altered by Th2 cytokines.
Journal:J Immunol
PubMed ID:9278322
'We sought to determine whether selected cytokines, known to profoundly increase proliferation and/or production of Ig by B cells and their progeny, also have as yet unrecognized effects upon IgA glycosylation. For these studies, we selected CH12LX mouse B lymphoma cells, a widely used model of B cell differentiation. Glycosylation
A knock-in mouse model of gastrointestinal stromal tumor harboring kit K641E.
Journal:Cancer Res
PubMed ID:16061643
A mouse model of gastrointestinal stromal tumor (GIST) has been developed by a knock-in gene targeting strategy, which introduced a Kit gene K641E mutation, originally identified in sporadic human GISTs and in the germ line of familial GIST syndrome patients. Homozygous and heterozygous Kit K641E mice develop gastrointestinal pathology with
Targeting the intracellular MUC1 C-terminal domain inhibits proliferation and estrogen receptor transcriptional activity in lung adenocarcinoma cells.
Journal:Mol Cancer Ther
PubMed ID:21862684
Mucin 1 (MUC1) is a diagnostic factor and therapy target in lung adenocarcinoma. MUC1 C-terminal intracellular domain (CD) interacts with estrogen receptor (ER) a and increases gene transcription in breast cancer cells. Because lung adenocarcinoma cells express functional ERa and ERß, we examined MUC1 expression and MUC1-ER interaction. Because blocking