Subcellular targeting of RGS9-2 is controlled by multiple molecular determinants on its membrane anchor, R7BP.
Authors:Song JH, Waataja JJ, Martemyanov KA,
Journal:J Biol Chem
PubMed ID:16574655
'RGS9-2, a member of the R7 RGS protein family of neuronal RGS (Regulators of G protein Signaling), is a critical regulator of G protein signaling. In striatal neurons, RGS9-2 is tightly associated with a novel palmitoylated protein - R7BP (R7 family Binding Protein). Here we report that R7BP acts to ... More
Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells.
Authors: Domi Arban; Moss Bernard;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12196634
'The ability to manipulate the vaccinia virus (VAC) genome, as a plasmid in bacteria, would greatly facilitate genetic studies and provide a powerful alternative method of making recombinant viruses. VAC, like other poxviruses, has a linear, double-stranded DNA genome with covalently closed hairpin ends that are resolved from transient head-to-head ... More
The regulated secretion and vectorial targeting of neurotrophins in neuroendocrine and epithelial cells.
Authors: Heymach J V Jr; Krüttgen A; Suter U; Shooter E M;
Journal:J Biol Chem
PubMed ID:8810312
'The varied roles that neurotrophins play in the development and activity-dependent plasticity of the nervous system presumably require that the sites and quantity of neurotrophin release be precisely regulated. As a step toward understanding how different neurotrophins are sorted and secreted by neurons, we expressed nerve growth factor (NGF), brain-derived ... More
Tyrphostins protect neuronal cells from oxidative stress.
Authors:Sagara Y, Ishige K, Tsai C, Maher P,
Journal:J Biol Chem
PubMed ID:12121989
'Tyrphostins are a family of tyrosine kinase inhibitors originally synthesized as potential anticarcinogenic compounds. Because tyrphostins have chemical structures similar to those of the phenolic antioxidants, we decided to test the protective efficacy of tyrphostins against oxidative stress-induced nerve cell death (oxytosis). Many commercially available tyrphostins, at concentrations ranging from ... More
The Plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.
Authors: Vikis Haris G; Li Weiquan; Guan Kun-Liang;
Journal:Genes Dev
PubMed ID:11937491
'The small GTPase Rac has been implicated in growth cone guidance mediated by semaphorins and their receptors. Here we demonstrate that plexin-B1, a receptor for Semaphorin4D (Sema4D), and p21-activated kinase (PAK) can compete for the interaction with active Rac and plexin-B1 can inhibit Rac-induced PAK activation. We have also demonstrated ... More