One Shot™ MAX Efficiency™ DH10B T1 噬菌体耐受细胞
One Shot™ MAX Efficiency™ DH10B T1 噬菌体耐受细胞
引用和文献 (2)
Invitrogen™

One Shot™ MAX Efficiency™ DH10B T1 噬菌体耐受细胞

DH10B T1 噬菌体耐受大肠杆菌具有 DH10B 基因型的所有优点，并添加了对 T1 和 T5 噬菌体感染产生耐受性的 tonA了解更多信息
Have Questions?
货号数量
1233101320 x 50μL
货号 12331013
价格（CNY)
2,243.00
Each
添加至购物车
数量:
20 x 50μL
价格（CNY)
2,243.00
Each
添加至购物车
DH10B T1 噬菌体耐受大肠杆菌具有 DH10B 基因型的所有优点，并添加了对 T1 和 T5 噬菌体感染产生耐受性的 tonA 基因型。tonA 基因型的加入保护了您的宝贵样品，并尽可能减少了因噬菌体污染而导致实验室停工的可能性。DH10B-T1R 基因型具有以下特点：

•对 T1 和 T5 噬菌体产生耐受性的 tonA 基因型
•消除 mcrA、mcrBC、mrr 和 hsdRMS 限制系统，以构建更具代表性的基因组文库 (1,2)
•endA1 突变可提高质粒得率和数量
ZΔM15 用于重组克隆的蓝白斑筛选

DH10B-T1R 大肠杆菌有电转感受态和化学感受态两种形式。高转化效率使其适用于制备 cDNA 或基因组文库。使用大小为 150 kb 的质粒可实现高效率转化 (3)。
仅供科研使用。不可用于诊断程序。
规格
耐抗生素细菌Yes (Streptomycin)
蓝色/白色筛查是
是否可克隆甲基化 DNA是
克隆不稳定 DNA不适合克隆不稳定DNA
是否含 F' 附加体缺乏 F’附加体
高通量能力不兼容高通量应用（手动）
提高质粒质量是
质粒高拷贝质粒
制备无甲基化 DNA不适合制备未甲基化DNA
产品线One Shot
产品类型感受态细胞
数量20 x 50μL
减少克隆重组现象是
运输条件Dry Ice
T1 噬菌体 - 抗性 (tonA)是
转化效率级别高效率 (> 10^9 cfu⁄μg)
产品规格One Shot
种属大肠杆菌
Unit SizeEach
内容与储存
储存于超低温冰箱（-68 至 -85°C）中。

常见问题解答 (FAQ)

你们提供Subcloning Efficiency、Library Efficiency和MAX Efficiency感受态细胞。它们的区别是什么？

有少数例外，但他们主要的区别在于所保证的转化效率：

Subcloning Efficiency细胞每转化1 µg pUC19或pUC18超螺旋质粒保证产生至少1.0 x 10E6个转化子。
Library Efficiency细胞每转化1 µg pUC19或pUC18 DNA保证产生至少1.0 x 10E8个转化子。
MAX Efficiency细胞每转化1 µg pUC19或pUC18 DNA保证产生至少1.0 x 10E9个转化子。

You offer competent cells in Subcloning Efficiency, Library Efficiency and MAX Efficiency. How do these differ?

There are a few exceptions, but in general the difference is in guaranteed transformation efficiency as follows:

Subcloning Efficiency cells are guaranteed to produce at least 1.0 x 10E6 transformants per µg of transformed pUC19 or pUC18 supercoiled plasmid
Library Efficiency cells are guaranteed to produce at least 1.0 x 10E8 transformants per µg pUC19 or pUC18 DNA
MAX Efficiency cells are guaranteed to produce at least 1.0 x 10E9 transformants per µg pUC19 or pUC18 DNA

引用和文献 (2)

引用和文献
Abstract
Calsarcin-3, a novel skeletal muscle-specific member of the calsarcin family, interacts with multiple Z-disc proteins.
Authors: Frey Norbert; Olson Eric N;
Journal:J Biol Chem
PubMed ID:11842093
'The Z-disc is a highly specialized multiprotein complex of striated muscles that serves as the interface of the sarcomere and the cytoskeleton. In addition to its role in muscle contraction, its juxtaposition to the plasma membrane suggests additional functions of the Z-disc in sensing and transmitting external and internal signals. ... More
Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA.
Authors:Amdam GV, Simões ZL, Guidugli KR, Norberg K, Omholt SW,
Journal:BMC Biotechnol
PubMed ID:12546706
BACKGROUND: The ability to manipulate the genetic networks underlying the physiological and behavioural repertoires of the adult honeybee worker (Apis mellifera) is likely to deepen our understanding of issues such as learning and memory generation, ageing, and the regulatory anatomy of social systems in proximate as well as evolutionary terms. ... More