在Stealth RNAi siRNA实验中我应该使用什么阳性对照和阴性对照？
我们为Stealth siRNA提供多个阳性和阴性对照：
Stealth阳性对照有：
- Stealth RNAi siRNA Actin阳性对照（人）（货号12935141, 250 µL）
- Stealth RNAi siRNA Cyclophilin B阳性对照（人）（货号12935142, 250 µL）
- Stealth RNAi siRNA GAPDH阳性对照（人）（货号12935140, 250 µL）
- Stealth RNAi siRNA β-Lactamase报告基因对照（货号12935148, 250 µL）
- Stealth RNAi siRNA GFP报告基因对照（货号12935145, 250 µL）
- Stealth RNAi siRNA Luciferase报告基因对照（货号12935146, 250 µL）
Stealth阴性对照有：
提供高(55-65% GC), 中(45-55% GC), 或低(35-45% GC)三种GC含量的双链siRNA对照：
- 高GC含量的Stealth RNAi siRNA阴性对照（货号12935400, 250 µL）
- 高GC含量的Stealth RNAi siRNA阴性对照#2（货号12935114, 250 µL）
- 高GC含量的Stealth RNAi siRNA阴性对照#3（货号12935115, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照（货号12935300, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照#2（货号12935112, 250 µL）
- 中GC含量的Stealth RNAi siRNA阴性对照#3（货号12935113, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照（货号12935200, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照#2（货号12935110, 250 µL）
- 低GC含量的Stealth RNAi siRNA阴性对照#3（货号12935111, 250 µL）
- Stealth RNAi siRNA阴性对照试剂盒（货号12935100，包含每种GC含量阴性对照各20 µM各250 µL）
- 对于随机序列阴性对照，您可以使用BLOCK-iT RNAi Designer对您的siRNA或Stealth RNAi序列进行打乱。
您也可以使用我们的BLOCK-iT AlexaFluorRed Fluorescent Control（货号14750100）或BLOCK-iTFluorescent Oligo（货号2013, 13750062）作为转染的对照。
What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?
We offer several negative and positive controls for Stealth siRNA:
For Stealth Positive Control:
- Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
- Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
- Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
- Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)
For Stealth Negative Control:
Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:
- Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
- Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
- For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.
You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Can I use the BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100)?
We do not recommend using BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100). BLOCK-iT Alexa Fluor Red Fluorescent Control (Cat. No. 14750100) should be used to assess transfection efficiency with Lipofectamine RNAiMAX Reagent.
BLOCK-iT Fluorescent Oligo, for lipid transfection is optimized for use with Lipofectamine 2000 Transfection Reagent and is not recommended for Lipofectamine RNAiMAX Reagent.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center
