Can the compatibility reagent in the BCA-RACE kit be stored after reconstitution and reused?
The reconstituted compatibility reagent is stable for 8 hours when stored at 4 degrees C protected from light.
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How many assays can be performed with each BCA-RAC kit?
The BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) has sufficient reagents for 250 samples or standards of 25µL each, and the Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) can be used to assay 1,000 samples or standards of 9µL each.
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Can I increase the volumes of the sample and compatibility reagent in the microplate assay when using the BCA-RAC?
No. Increasing the sample and compatibility reagent volumes will require increasing the BCA working reagent, resulting in a volume that exceeds the well capacity. There must be space remaining in the well to allow mixing and prevent the solution from contacting the sealing tape.
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What formats are available for the BCA-RAC?
There are two different formats: the BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) for microcentrifuge tube (1.5ml) assays and Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) for 96-well plates. The tube assay is compatible with 5mM DTT, 10mM TCEP and 35mM 2ME, and the microplate assay is compatible with 5mM DTT, 10mM TCEP and 25mM 2ME.
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How does the BCA-RAC compare with the original BCA assay?
The BCA-RAC provides the advantages of the original BCA assay (compatibility with detergents, buffer/salts and solvents) combined with reducing agent compatibility at concentrations routinely used in protein sample buffers. The BCA assay is based on reduction of Cu+2 to Cu+1 by proteins in an alkaline solution and the subsequent complexing of the Cu+1 to BCA reagent, yielding a purple color detectable at 562nm. Disulfide reducing agents such as DTT, 2ME and TCEP are also capable of reducing Cu+2 to Cu+1. To minimize their reducing effect, a compatibility reagent that modifies disulfide reducing agents is added to the sample before performing the assay.
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