Pierce™ 谷胱甘肽包被板
Pierce™ 谷胱甘肽包被板
Thermo Scientific™

Pierce™ 谷胱甘肽包被板

Thermo Scientific™ Pierce 谷胱甘肽包被板是标准 96 孔微孔板,使用完整谷胱甘肽 (GSH) 进行了包被,并进行了预封闭,以用于涉及谷胱甘肽-S-转移酶 (GST) 融合蛋白的捕获和检测试验。
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货号颜色产品规格检测方法
15140清除单条可拆比色法
15340黑色Standard荧光
15240白色Standard化学发光
货号 15140
价格(CNY)
4,495.00
Each
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颜色:
清除
产品规格:
单条可拆
检测方法:
比色法
请求批量或定制报价
价格(CNY)
4,495.00
Each
添加至购物车
Pierce 谷胱甘肽包被板使用完整谷胱甘肽 (GSH) 进行了包被,并进行了预封闭,以用于涉及谷胱甘肽-S-转移酶 (GST) 融合蛋白的捕获和检测试验。这些包被板可用于细胞裂解物分析,以确定表达系统中产生的 GST 融合蛋白的存在情况和浓度。也可利用试剂盒用 GST 标签捕获和包被融合蛋白,用于涉及重组蛋白的后续结合测定。由于板经过预封闭,不需要对细胞裂解物进行初始纯化。
Thermo Scientific Pierce 谷胱甘肽包被板是微孔板,使用完整谷胱甘肽 (GSH) 进行了包被,并进行了预封闭,以用于涉及谷胱甘肽-S-转移酶 (GST) 融合蛋白的捕获和检测试验。

谷胱甘肽包被板的特点包括:
选择性—特异性结合含谷胱甘肽 S 转移酶的融合蛋白
容量—结合约 10 ng GST 蛋白/孔
通用—随时可结合的板形式可用于蛋白相互作用研究
兼容—用于裂解细胞的去污剂不会抑制 GST 蛋白与预包被板的结合(与使用聚苯乙烯板进行直接包被的方法相比)
即用型—使用 BSA 和 SuperBlock 封闭缓冲液进行预封闭以减少非特异性结合

仅供科研使用。不可用于诊断程序。
规格
结合属性标准容量,亲和
检测方法比色法
包装5块板
板封闭SuperBlock™ 缓冲液
产品类型塑料、无涂层、非无菌孔板
数量5 plates
颜色清除
产品规格单条可拆
材质聚苯乙烯
孔数量96
产品线Pierce
表面处理谷胱甘肽
Unit SizeEach
内容与储存
在 4°C 下储存在未开封的袋中。开封后,将未使用的微孔板储存在 4°C 下可重复密封的含干燥剂的袋中。

常见问题解答 (FAQ)

How does Penicillin work?

Penicillin interferes with the final stages of bacterial cell wall synthesis.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the detection limit of proteins with Pierce Glutathione Coated Plates?

The background is extremely low when using the Pierce Glutathione Coated Plates. This results in a high signal-to-noise ratio and a low detection limit which in general means that the detection limit (OD 0.5) is 3 ng per well (100 µL).

How long are Pierce Glutathione Coated Plates stable?

The Pierce Glutathione Coated Plates are extremely stable over long periods of time, if stored at room temperature (20-25 degrees C). We guarantee 12 mths of shelf life in unopened foil package after the date of shipment.

Can I determine the concentration of an unknown GST-tagged protein?

To determine the approximate concentration of a GST-tagged protein, a standard curve should be generated using purified GST added in different concentrations to the wells of the plates. Please note that it is only the approximately concentration that can be determined since not 100% of the GST-fusion proteins will be bound due to denaturing or steric hindrance to GSH.

Can I whirl-mix solutions containing GST proteins?

No. Do not whirl-mix a solution containing GST-tagged proteins. The CV will increase due to slight degradation of the GST protein.