胰蛋白酶-EDTA (0.5%),无酚红
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胰蛋白酶-EDTA (0.5%),无酚红
胰蛋白酶-EDTA (0.5%),无酚红
胰蛋白酶-EDTA (0.5%),无酚红
Gibco™

胰蛋白酶-EDTA (0.5%),无酚红

Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive了解更多信息
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货号数量
15400054100 mL
货号 15400054
价格(CNY)
673.00
Each
添加至购物车
数量:
100 mL
Customize this product
价格(CNY)
673.00
Each
添加至购物车
Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. Concentrated forms of trypsin can be aseptically diluted to 1X using a balanced salt solution without calcium and magnesium. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE reagent that feature:
• Quality testing
• Documented traceability
• Dual-site cGMP Manufacturing

This trypsin is modified as follows:

With
• EDTA

Without
• Phenol Red

The complete formulation is available.

Quality testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco Trypsin-EDTA (0.5%), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
仅用于研究和生产用途。不可用于诊断程序。
规格
细胞类型哺乳动物细胞
螯合剂EDTA
最大浓度10X
渗透压300 - 345 mOsm/kg
产品线Gibco™
数量100 mL
有效期18 个月
运输条件湿冰
来源动物来源
经过测试体外生物测定
分类动物来源
形式液体
产品类型细胞培养解离试剂
无菌无菌过滤
加有添加剂EDTA
不加添加剂无酚红
pH7.1 - 8.0
Unit SizeEach
内容与储存
储存条件:-5°C 至 -20°C
有效期:自生产之日起 18 个月

常见问题解答 (FAQ)

解冻后的胰酶可在冰箱中放置多长时间?

通常情况下,胰酶可在冰箱中保存一周。不过,这一时间长度也随着这一周内的加热次数和使用频率而变化。同时,它也与细胞粘附的紧密程度有关,胰酶在非冰冻条件下每天都会丧失一定的活性。

Gibco TrypLE试剂与胰酶相比优势在于?

TrypLE试剂不含动物源性与人源成份,纯度极高且对细胞的作用十分温和。该产品无需使用胰酶抑制剂对其进行终止。在含血清和不含血清的培养条件下,TrypLE试剂都是解离各类贴壁培养的哺乳动物细胞系的理想之选,能够直接做为胰酶替代品而应用于您当前的实验方案。

TrypLE试剂在常温下十分稳定,可随时按需取用。TrypLE细胞解离试剂能够在室温下稳定保存24个月,储存与操作都更为容易方便。

你们提供哪些用于解离细胞的试剂?这些产品之间的主要区别是什么?

请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。

你们是否提供不含酚红的培养基(无酚红培养基)?

是的,请访问此页面(https://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf)浏览我们的无酚红培养基页面。

I received Trypsin-EDTA (0.5%), no phenol red (Cat. No. 15400054) with ice packs instead of dry ice and the product was partially melted. Is it OK to use?

Trypsin is shipped on wet ice as the shipment on dry ice can cause changes in pH which influences the product quality negatively. If you receive the trypsin thawed or partially thawed, the stability and functionality of the product will not be impacted. For further information please see the white paper at the following link.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.