克隆的核糖核酸酶抑制剂以 10,000 单位/ml 的浓度提供,溶剂为 20 mM HEPES-KOH(pH 值 7.6)、50 mM KCl、8 mM DTT 和 50% (v/v) 甘油。保存于 -20°C。
引用和文献 (3)
引用和文献
Abstract
Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae.
Authors: Burchett Scott A; Flanary Paul; Aston Christopher; Jiang Lixin; Young Kathleen H; Uetz Peter; Fields Stanley; Dohlman Henrik G;
Journal:J Biol Chem
PubMed ID:11940600
'All members of the regulator of G protein signaling (RGS) family contain a conserved core domain that can accelerate G protein GTPase activity. The RGS in yeast, Sst2, can inhibit a G protein signal leading to mating. In addition, some RGS proteins contain an N-terminal domain of unknown function. Here ... More
Involvement of an upstream stimulatory factor as well as cAMP-responsive element-binding protein in the activation of brain-derived neurotrophic factor gene promoter I.
Authors:Tabuchi A, Sakaya H, Kisukeda T, Fushiki H, Tsuda M
Journal:J Biol Chem
PubMed ID:12114522
'The use of different brain-derived neurotrophic factor (BDNF) gene promoters results in the differential production of 5''-alternative transcripts, suggesting versatile functions of BDNF in neurons. Among four BDNF promoters I, II, III, and IV (BDNF-PI, -PII, -PIII, and -PIV), BDNF-PI was markedly activated, as well as BDNF-PIII, by Ca(2+) signals ... More
Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid.
Authors: Lu C; Han M-H; Guevara-Garcia A; Fedoroff N V;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12434021
Abscisic acid (ABA) mediates plant responses to environmental stress, particularly to water status. During germination, the embryo emerges from dormancy as the ABA concentration declines. Exposure to exogenous ABA during germination arrests development rapidly, but reversibly, enabling seedlings to withstand early water stress without loss of viability. Postgermination proteolytic degradation ... More