Restore™ Western 印迹剥离缓冲液
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Restore™ Western 印迹剥离缓冲液
Thermo Scientific™

Restore™ Western 印迹剥离缓冲液

Thermo Scientific Restore Western Blot抗体剥离缓冲液能够安全高效地从硝化纤维膜和PVDF膜上清去除一抗和二抗,从而再次检测化学发光的蛋白质免疫印迹。estore Western Blot抗体剥离缓冲液的特性:•省时—不需重新制胶电泳•节省珍贵样品—在同一张膜上使用相同的样品重新检测•高效—专利配方,去除效果好远优于自制缓冲液•温和配方—在抗体剥离及重新检测时不会对靶蛋白造成伤害•无气味—不含硫醇,无刺鼻气味•价格经济—比其他竞争厂商的剥离缓冲液价格更低产品细节进行凝胶电泳并使用平行双样免疫印迹分析来检测新的一抗或抗体浓度非常耗费时间和金钱,而Restore Western Blot抗体剥离缓冲液可以消除使用化学发光免疫印迹底物检测免疫印迹过程中的浪费了解更多信息
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货号数量
210635 L
2106230 mL
21059500 mL
货号 21063
价格(CNY)
8,937.00
Each
添加至购物车
数量:
5 L
请求批量或定制报价
价格(CNY)
8,937.00
Each
添加至购物车
Thermo Scientific Restore Western Blot抗体剥离缓冲液能够安全高效地从硝化纤维膜和PVDF膜上清去除一抗和二抗,从而再次检测化学发光的蛋白质免疫印迹。

estore Western Blot抗体剥离缓冲液的特性:

•省时—不需重新制胶电泳
•节省珍贵样品—在同一张膜上使用相同的样品重新检测
•高效—专利配方,去除效果好远优于自制缓冲液
•温和配方—在抗体剥离及重新检测时不会对靶蛋白造成伤害
•无气味—不含硫醇,无刺鼻气味
•价格经济—比其他竞争厂商的剥离缓冲液价格更低

产品细节
进行凝胶电泳并使用平行双样免疫印迹分析来检测新的一抗或抗体浓度非常耗费时间和金钱,而Restore Western Blot抗体剥离缓冲液可以消除使用化学发光免疫印迹底物检测免疫印迹过程中的浪费。Restore 抗体剥离缓冲液可以从免疫印迹中彻底、高效地从去除一抗和二抗,而不会去除或破坏已固定抗原,从而使得印迹可以被清除,而抗原可以重新检测,结果依然可靠。

使用Thermo Scientific SuperSignal辣根过氧化物酶底物进行化学发光免疫印迹检测,是当前最常见、最灵敏的方法。由于这些底物不会沉淀并与膜表面永久结合,因此化学发光法检测到的免疫蛋白印迹可使用该试剂来剥离,从而移除亲和结合的一抗和二抗。为了达到良好的效果,抗体剥离缓冲液需要具有足够的强度来分离已结合的抗体,还需要足够温和使已转移的标靶蛋白仍保留在硝化纤维膜或PVDF膜上。Restore Western Blot 抗体剥离正具有这些特点。

通过剥离和重新检测,我们无需为了对不同标靶进行检测而进行多次凝胶电泳,避免浪费稀少或昂贵的样品。一次凝胶电泳产生的一张膜可通过使用Restore Western Blot抗体剥离缓冲液来去除一抗从而消除印迹。印迹剥离仅需花费15到30分钟,具体时间因一抗的亲和能力而不同。在去除印迹后,可使用新的一抗重新结合并检测。另外,印迹剥离之后还可以使用调整后的抗体浓度来进行重新结合,以便在初次结果不佳的情况下进一步优化实验条件。

应用
•可重复使用使用硝化纤维膜或PVDF膜,通过不同的一抗检测不同标靶
•可重新检测印迹,改正或优化初次实验中无效的抗体浓度

实验方案概要
•漂洗印迹以去除化学发光底物。
•37°C(对于某些抗体室温即可)条件下,在Restore Western Blot抗体剥离缓冲液中孵育印迹5至15分钟。
•移除免疫印迹并在洗涤缓冲液(TBS-T或PBS-T)中进行清洗。
•测试抗体是否已充分移除。
•进行下一次免疫印迹实验。
仅供科研使用。不可用于诊断程序。
规格
检测方法化学发光
适用于(应用)蛋白质免疫印迹
形式液体
膜兼容性硝酸纤维素、PVDF
产品线Restore
产品类型Western 印迹剥离缓冲液
数量5 L
Unit SizeEach
内容与储存
储存:接收后在室温或 4°C 下储存。产品在环境温度下运输。

常见问题解答 (FAQ)

How many times can I strip my blot using Restore or Restore Plus Western Blot Stripping Buffers ?

The stability of the attached (transferred and bound) protein will determine the number of times the membrane can be successfully re-probed after stripping. The protein may withstand stripping as many as four times or as few as one time.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the proper wash buffer to use after stripping with Restore or Restore Plus Western Blot Stripping buffers?

Wash the membrane with the same buffer as was used between antibody probing steps during the Western blotting procedure.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I strip membranes with Restore or Restore Plus Western Blot Stripping Buffer and reprobe for subsequent detection with fluorescent probes?

No. Although the fluorescent antibodies, like other antibodies, can be stripped with Restore Buffers, stripped membranes typically produce unacceptable background for subsequent fluorescent detection methods. We recommend Restore Fluorescent Western Blot Stripping Buffer, Cat. No. 62299 (20 mL) and Cat. No. 62300 (100 mL).

Note: Restore Fluorescent Western Blot Stripping Buffer is for use with low-fluorescence PVDF membrane (Cat. No. 22860) only.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will Restore and Restore Plus Western Blot Stripping Buffers remove biotin-bound avidin, streptavidin or NeutrAvidin Protein conjugates?

No. Restore and Restore Plus Buffers are not stringent enough to break the avidin-biotin interaction. A buffer capable of breaking this interaction would likely also damage the target protein, making it undetectable. However it may be possible to strip the complexed biotinylated primary antibody and streptavidin-HRP secondary antibody from the target protein on the membrane. Please also see Tech Tip: Strip and Reprobe Western Blots (https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/strip-reprobe-western-blots-tech-tip.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will the Restore and Restore Plus Western Blot Stripping Buffers remove precipitating substrates?

No. The antibodies are removed but the substrate leaves a permanent precipitate on the membrane that cannot be removed. Restore and Restore Plus Buffers are designed for procedures using chemiluminescent substrates. Please note that this is not compatible with fluorescence supstrates as it will result in increased background. For fluorescent substrates please use our Restore Fluorescent Western Blot Stripping Buffer. Please also see Tech Tip: Strip and Reprobe Western Blots (https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/strip-reprobe-western-blots-tech-tip.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.