Pierce™ 生物素化蛋白相互作用 Pull-Down 试剂盒
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Pierce™ 生物素化蛋白相互作用 Pull-Down 试剂盒
Thermo Scientific™

Pierce™ 生物素化蛋白相互作用 Pull-Down 试剂盒

Thermo Scientific Pierce 生物素化蛋白相互作用沉降试剂盒包含捕获和纯化生物素标记蛋白或配体相互作用体的必要组分。生物素化蛋白相互作用沉降试剂盒的特点：•为发现蛋白:蛋白相互作用提供一种完整、经济且易于使用的策略• 使用常用实验室设备和试剂（例如微量离心机、微型凝胶、蛋白染料了解更多信息
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货号数量
211151 个试剂盒
货号 21115
价格（CNY)
6,102.00
飞享价
Ends: 31-Dec-2025
8,258.00
共减 2,156.00 (26%)
Each
添加至购物车
数量:
1 个试剂盒
请求批量或定制报价
价格（CNY)
6,102.00
飞享价
Ends: 31-Dec-2025
8,258.00
共减 2,156.00 (26%)
Each
添加至购物车
Thermo Scientific Pierce 生物素化蛋白相互作用沉降试剂盒包含捕获和纯化生物素标记蛋白或配体相互作用体的必要组分。

生物素化蛋白相互作用沉降试剂盒的特点：

•为发现蛋白:蛋白相互作用提供一种完整、经济且易于使用的策略
• 使用常用实验室设备和试剂（例如微量离心机、微型凝胶、蛋白染料）
• 适合单份或多份样品需求
• 灵活沉降形式使用离心杯方便有效地操作链霉素亲和素琼脂糖微珠

您提供生物素化蛋白作为“诱饵”（请参见

相关产品
EZ-Link™ 脱硫生物素化和沉降试剂盒
仅供科研使用。不可用于诊断程序。
规格
检测亲和纯化
适用于（应用）沉降（基于生物素）
适用于（设备）小型离心机
产品规格试剂盒
数量1 个试剂盒
足够用于25 次反应
靶标无靶标特异性
产品线Pierce™
类型沉降试剂盒
Unit SizeEach
内容与储存
足够用于：至少进行 25 次沉降反应，各反应均使用 25 μL 树脂
• 链霉素亲和素琼脂糖树脂，1.5 mL
• TBS 包（制成 500mL），1 包
• 生物素封闭溶液，15 mL
• 洗涤缓冲液（pH 值 5.0），100 mL
• 洗脱缓冲液（pH 值 2.8），50 mL
• Pierce 离心柱和附件，27 柱
• 微量离心机收集管，200 管

在4°C 下储存

常见问题解答 (FAQ)

If my protein is endogenously expressed, which Thermo Scientific Protein:Protein Interaction (PPI) kit should I choose?

We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How is the tagged protein detected on the membrane using the Thermo Scientific Protein:Protein Interaction (PPI) kits?

The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are all of the conditions for binding the prey protein to the bait protein described in the Thermo Scientific Protein:Protein Interaction (PPI) kits?

No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ 生物素化蛋白相互作用 Pull-Down 试剂盒 FAQs