Pierce™ His 蛋白相互作用 Pull-Down 试剂盒
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Pierce™ His 蛋白相互作用 Pull-Down 试剂盒
Thermo Scientific™

Pierce™ His 蛋白相互作用 Pull-Down 试剂盒

Thermo Fisher Scientific Pierce His 标签蛋白相互作用沉降试剂盒包含捕获并纯化与 His 标签融合蛋白相互作用的蛋白所需的组分。His 标签蛋白相互作用沉降试剂盒的特点:•6xHis了解更多信息
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货号数量
212771 个试剂盒
货号 21277
价格(CNY)
5,087.00
飞享价
Ends: 31-Dec-2025
6,884.00
共减 1,797.00 (26%)
Each
添加至购物车
数量:
1 个试剂盒
价格(CNY)
5,087.00
飞享价
Ends: 31-Dec-2025
6,884.00
共减 1,797.00 (26%)
Each
添加至购物车
Thermo Fisher Scientific Pierce His 标签蛋白相互作用沉降试剂盒包含捕获并纯化与 His 标签融合蛋白相互作用的蛋白所需的组分。

His 标签蛋白相互作用沉降试剂盒的特点:

6xHis 沉降(产品编号 21277)—纯化任何 His 标签融合蛋白的蛋白相互作用体
全套试剂盒—可提供纯化蛋白:蛋白相互作用的所有组分和详细方案
无需特殊设备—使用常用实验室设备和试剂(例如,微量离心机)
便利—微量离心柱促进琼脂糖微珠的简单高效操作,包括简单处理多份样品
灵活—指示包括与各种样品类型表达的诱饵和猎物蛋白配合使用的方案
非特异性结合较少—与镍树脂相比,钴螯合树脂对组氨酸标签融合蛋白更具特异性,产生较少的非特异性结合
结合— 10 至 25 mg 组氨酸标签融合蛋白/mL 树脂

应用:

•发现细胞裂解物的一种新蛋白:蛋白相互作用的蛋白
•确认来自细胞裂解物或先前纯化的蛋白的假定相互作用
•从体外转录/翻译裂解物中提取蛋白:蛋白相互作用信息

您提供标签融合蛋白作为“诱饵”和表达假定蛋白相互作用靶标(“猎物”)的细胞,沉降试剂盒提供其他:细胞裂解缓冲液、微量离心柱、标签特异性亲和树脂(琼脂糖微珠)和优化缓冲液和方案。沉降试剂盒设计用于向初次用户教授该方法并提高经验丰富的研究人员的易用性、便利性和可重现性。

相关产品
Pierce™ GST 蛋白相互作用沉降试剂盒
仅供科研使用。不可用于诊断程序。
规格
检测亲和纯化
适用于(应用)沉降(基于标签)
适用于(设备)小型离心机
产品规格试剂盒
数量1 个试剂盒
足够用于25 次反应
靶标无靶标特异性
产品线Pierce™
类型沉降试剂盒
Unit SizeEach
内容与储存
足够用于:至少 25 次沉降反应,每次使用 25 μL 树脂
•钴螯合琼脂糖树脂,750 μL
•沉降裂解缓冲液,250 mL
•咪唑溶液 (4 M)、5 mL
• TBS 包(制备为 500 mL),1 包
• Pierce 离心柱和配件,27 个柱
•微量离心机收集管,100 个管

接收后,在室温下储存 Pierce 裂解缓冲液。在 4°C 下储存剩余的试剂盒组分。

常见问题解答 (FAQ)

If my protein is endogenously expressed, which Thermo Scientific Protein:Protein Interaction (PPI) kit should I choose?

We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How is the tagged protein detected on the membrane using the Thermo Scientific Protein:Protein Interaction (PPI) kits?

The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are all of the conditions for binding the prey protein to the bait protein described in the Thermo Scientific Protein:Protein Interaction (PPI) kits?

No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.