SPDP（琥珀酰亚胺 3-(2-吡啶基二硫基)-丙酸酯）
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SPDP（琥珀酰亚胺 3-(2-吡啶基二硫基)-丙酸酯）
引用和文献 (6)
Thermo Scientific™

SPDP（琥珀酰亚胺 3-(2-吡啶基二硫基)-丙酸酯）

Thermo Scientific Pierce SPDP 是一种短链交联剂，可以通过 NHS 酯和吡啶基二硫基反应性基团与半胱氨酸巯基形成可切割（可还原）二硫键，实现胺与巯基的偶联。SPDP了解更多信息
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货号数量
2185750 mg
货号 21857
价格（CNY)
2,655.00
飞享价
Ends: 31-Dec-2025
3,389.00
共减 734.00 (22%)
Each
添加至购物车
数量:
50 mg
请求批量或定制报价
价格（CNY)
2,655.00
飞享价
Ends: 31-Dec-2025
3,389.00
共减 734.00 (22%)
Each
添加至购物车
Thermo Scientific Pierce SPDP 是一种短链交联剂，可以通过 NHS 酯和吡啶基二硫基反应性基团与半胱氨酸巯基形成可切割（可还原）二硫键，实现胺与巯基的偶联。

SPDP 的特点：

•反应性基团：NHS 酯和吡啶基二硫基
•可与以下基团发生反应：氨基和巯基
•与游离巯基反应时释放可检测的副产物；通过测量 343 nm 处 2-巯基吡啶的释放，可轻松跟踪反应
•间隔臂中的二硫键可使用 10-50 mM DTT 或 TCEP（pH 值 8.5）轻松切割
•间隔臂也可使用还原 SDS-PAGE 上样缓冲液轻松切割
•可切割交联剂能够实现交联产物的分离
•不溶于水（首先溶于 DMF 或 DMSO）
•SPDP 交联剂具有膜可透过性，可在细胞内完成交联
•与其他多种 SPDP 型试剂具有可比性，包括聚乙二醇化形式

应用：
•蛋白的可逆交联
•通过连接至赖氨酸残基向蛋白添加巯基
•通过两步反应蛋白交联形成抗体-酶和其他生物偶联物
•半抗原连接至载体蛋白用于抗体生产
•制备免疫毒素偶联物

规格：
我们按照较高规格生产 SPDP，产生高特异性的生物偶联物，确保数据的完整性并提供较高程度的一致性。每批 SPDP 经过定量 NMR（交联剂纯度的较高标准）检测，确保纯度 >95%。

我们提供三种 SPDP 类似物：标准形式 (SPDP)、间隔臂较长的衍生物 (LC-SPDP) 和磺化水溶性变体 (Sulfo-LC-SPDP)。SPDP（N-琥珀酰亚胺 3-(2-吡啶基二硫基)-丙酸酯）和 LC-SPDP（琥珀酰亚胺 6-(3-[2-吡啶基二硫代]-丙酰氨基)已酸酯）是异型双功能、硫醇可切割和膜可透过性交联剂。它们各自含有一个胺反应性 N-羟基琥珀酰亚胺 (NHS) 酯，与赖氨酸残基反应形成稳定的酰胺键。间隔臂的另一端以吡啶二硫基团终止，与巯基反应形成可逆二硫键。

产品参考：
交联剂应用指南 -- 检索本产品的最新参考文献

相关产品
Pierce™ 优级 SPDP
LC-SPDP（琥珀酰亚胺6-(3-(2-吡啶基二硫代)丙酰氨基)已酸酯）
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性是
描述SPDP
形式粉末
标记方法化学标记
分子量312.36
聚乙二醇化否
产品线Pierce
数量50 mg
反应一部分NHS 酯、吡啶二硫化物
运输条件环境
溶解度DMF、DMSO
间隔臂长6.8 Å
水溶性否
化学反应性胺-巯基
可裂解用硫醇
交联剂类型异型双功能团试剂
产品规格标准、特优级
产品类型交联剂
间隔子短 (<10 Å)
Unit SizeEach
内容与储存
接收后，在 -20°C 下防潮储存。

常见问题解答 (FAQ)

Can you provide the shelf-life for SPDP (succinimidyl 3-(2-pyridyldithio)propionate)?

SPDP (succinimidyl 3-(2-pyridyldithio)propionate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用和文献 (6)

引用和文献
Abstract
Development and characterization of a potent immunoconjugate targeting the Fn14 receptor on solid tumor cells.
Authors:Zhou H, Marks JW, Hittelman WN, Yagita H, Cheung LH, Rosenblum MG, Winkles JA,
Journal:Mol Cancer Ther
PubMed ID:21586630
'TNF-like weak inducer of apoptosis (TWEAK) and fibroblast growth factor (FGF)-inducible 14 (Fn14) are a TNF superfamily ligand-receptor pair involved in many cellular processes including proliferation, migration, differentiation, inflammation, and angiogenesis. The Fn14 receptor is expressed at relatively low levels in normal tissues, but it is known to be dramatically ... More
Evidence for the assembly of a bacterial tripartite multidrug pump with a stoichiometry of 3:6:3.
Authors:Janganan TK, Bavro VN, Zhang L, Matak-Vinkovic D, Barrera NP, Venien-Bryan C, Robinson CV, Borges-Walmsley MI, Walmsley AR
Journal:J Biol Chem
PubMed ID:21610073
'The multiple transferable resistance (mTR) pump from Neisseria gonorrhoeae MtrCDE multidrug pump is assembled from the inner and outer membrane proteins MtrD and MtrE and the periplasmic membrane fusion protein MtrC. Previously we established that while there is a weak interaction of MtrD and MtrE, MtrC binds with relatively high ... More
Winding single-molecule double-stranded DNA on a nanometer-sized reel.
Authors:You H, Iino R, Watanabe R, Noji H
Journal:Nucleic Acids Res
PubMed ID:22772992
A molecular system of a nanometer-sized reel was developed from F(1)-ATPase, a rotary motor protein. By combination with magnetic tweezers and optical tweezers, single-molecule double-stranded DNA (dsDNA) was wound around the molecular reel. The bending stiffness of dsDNA was determined from the winding tension (0.9-6.0 pN) and the diameter of ... More
Quantitative ratiometric discrimination between noncancerous and cancerous prostate cells based on neuropilin-1 overexpression.
Authors:Pallaoro A, Braun GB, Moskovits M
Journal:Proc Natl Acad Sci U S A
PubMed ID:21930955
A multiplexed, ratiometric method is described that can confidently distinguish between cancerous and noncancerous epithelial prostate cells in vitro. The technique is based on bright surface-enhanced resonance Raman scattering (SERRS) biotags (SBTs) infused with unique Raman reporter molecules, and carrying cell-specific peptides. Two sets of SBTs were used. One targets ... More
A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps.
Authors:Lobedanz S, Bokma E, Symmons MF, Koronakis E, Hughes C, Koronakis V
Journal:Proc Natl Acad Sci U S A
PubMed ID:17360572
Bacteria such as Escherichia coli and Pseudomonas aeruginosa expel antibiotics and other inhibitors via tripartite multidrug efflux pumps spanning the inner and outer membranes and the intervening periplasmic space. A key event in pump assembly is the recruitment of an outer membrane-anchored TolC exit duct by the adaptor protein of ... More
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