2-巯基乙醇

Gibco™ 2-巯基乙醇（也称为 β-巯基乙醇或 BME）是细胞培养基中用于防止氧自由基中毒的有效还原剂。2-巯基乙醇在溶液中并不稳定。因此，大多数方案都需要每日补充。Gibco 2-巯基乙醇在了解更多信息

货号	数量
21985023	50 mL

货号 21985023

价格（CNY)

912.00

添加至购物车

50 mL

价格（CNY)

912.00

添加至购物车

Gibco™ 2-巯基乙醇（也称为 β-巯基乙醇或 BME）是细胞培养基中用于防止氧自由基中毒的有效还原剂。2-巯基乙醇在溶液中并不稳定。因此，大多数方案都需要每日补充。Gibco 2-巯基乙醇在 Dulbecco's 磷酸盐缓冲液 (DPBS) 中含浓度为 55 mM 的 2-巯基乙醇。

cGMP 生产和质量体系
Gibco 还原剂在位于纽约格兰德岛上符合 cGMP 要求的工厂内生产。该公司是通过FDA 登记的医疗器械生产商以及通过 ISO 13485 标准认证.

仅供科研使用。不可用于诊断程序。

细胞类型哺乳动物细胞

最大浓度55 mM

数量50 mL

有效期36 个月

运输条件室温

产品类型BME

pH6 - 8

Unit SizeEach

内容与储存

储存条件：2°C 至8°C
运输条件：室温
有效期：自生产之日起36个月

常见问题解答 (FAQ)

I've purchased Gibco 2-Mercaptoethanol (Cat. No. 21985023). Should I add it to my cell culture medium right before every use or can I add it to the bulk medium in advance?

The 2-Mercaptoethanol must be added to the medium immediately before use because activity rapidly declines in diluted form. You can supplement enough medium needed for that day.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (2)

引用和文献

Abstract

Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.

Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,

Journal:Proc Natl Acad Sci U S A

PubMed ID:11248053

'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More

Somatic coding mutations in human induced pluripotent stem cells.

Authors:Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K,

Journal:Nature

PubMed ID:21368825

Defined transcription factors can induce epigenetic reprogramming of adult mammalian cells into induced pluripotent stem cells. Although DNA factors are integrated during some reprogramming methods, it is unknown whether the genome remains unchanged at the single nucleotide level. Here we show that 22 human induced pluripotent stem (hiPS) cell lines ... More