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Yes. All detergents and even phospholipids in cell extracts will form mixed micelles with SDS and migrate down into the gel. They can also interfere with the SDS:protein binding equilibrium. Most of the nonionic detergents significantly interfere with SDS-PAGE. We recommend that you keep the ratio of SDS to lipid or other detergent at 10:1 (or greater) to minimize these effects.Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.