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Thermo Scientific™
Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
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| 货号 | 数量 |
|---|---|
| 32132 | 100 mL Kit |
| 32134 | 25 mL Kit |
| 32132X3 | 300 mL Kit |
货号 32132
价格（CNY)
3,917.00
Each
数量:
100 mL Kit
价格（CNY)
3,917.00
Each
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
- Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
- Higher sensitivity—detect targets down to the low-picogram level
- Longer signal duration—sustained light output for as long as 5 hours
- More imaging options—X-ray, CCD or laser-based imagers
- More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
仅供科研使用。不可用于诊断程序。
规格
偶联物HRP
描述Pierce ECL Plus Western Blotting Substrate
套装内容Detection Reagent A, 100 mL, Detection Reagent B, 2.5 mL
灵敏度Low picogram to mid-femtogram
足够用于10 mini-gel blots, 1000 cm2 of membrane
检测方法Chemiluminescence, Fluorescence
适用于（应用）Protein Assay and Analysis
形式Liquid
膜兼容性Nitrocellulose, PVDF
产品线Pierce
产品类型底物
数量100 mL Kit
Unit SizeEach
内容与储存
Upon receipt store at 4°C. Product shipped on ice.