Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP了解更多信息
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货号
数量
34083
500 mL
货号 34083
价格(CNY)
6,164.00
Each
添加至购物车
数量:
500 mL
请求批量或定制报价
价格(CNY)
6,164.00
Each
添加至购物车
Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP conjugate for the detection of rabbit primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.
Features of SuperSignal West Pico Complete Rabbit IgG Detection Kit:
• Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions • Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane) • Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer
All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.
足够用于:采用兔一抗探测的 ∼62 个微型印迹 • SuperSignal West Pico 鲁米诺/增强剂溶液,250 mL • SuperSignal West Pico 稳定过氧化物溶液,250 mL • 过氧化物酶偶联的山羊抗兔 IgG (H + L),2 mL
常见问题解答 (FAQ)
How can I increase the sensitivity of my Western blot?
Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.