序列检测系统光谱校准工具包（96 孔）

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR® Green, TAMRA™,了解更多信息

货号	数量
4328639	1 x 96 孔板

货号 4328639

价格（CNY)

10,848.00

添加至购物车

1 x 96 孔板

价格（CNY)

10,848.00

添加至购物车

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR® Green, TAMRA™, TET™, and VIC™ dyes on the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System. The Sequence Detection Systems Spectral 96-Well Calibration kit includes three optical 96-well reaction plates: one preloaded and sealed background plate, and two preloaded and sealed spectral calibration plates with eight separate dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™,ROX™, SYBR® Green , TAMRA™, TET™, and VIC™) are provided for quick and easy calibration of the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

仅供科研使用。不可用于诊断程序。

已校准染料FAM, JOE, NED, ROX, TAMRA™, TET™, VIC, SYBR™ Green

校准类型光谱校准

描述96 孔板

检测方法荧光

适用于（应用）实时 PCR (qPCR)

适用于（设备）7900HT 系统

数量1 x 96 孔板

运输条件干冰

产品类型光谱校准试剂盒

Unit SizeEach

内容与储存

包含一个背景板和两个光谱校准板（包含 8 种染料标准品(FAM™、JOE™、NED™、ROX™、SYBR™ Green、TAMRA™、TET™、VIC™)）。

在 -15 到 25°C 下储存。避光。

有效期：自交货日期起 12 个月

常见问题解答 (FAQ)

How can I convert a file from AQ to RQ, or from RQ to AQ file with the 7900HT Fast Real-Time PCR System?

SDS v2.4 and above allow you to convert your AQ files to RQ and vice versa.
To do so, simply go to: Tools then AQ RQ Converter
-Choose from the dropdown which direction you want to go (AQ to RQ, or RQ to AQ).
-Next click on ‘Add' and browse to the plate file location in the new window. and click ‘Open'.
-Click on ‘Convert'. A new file will be created in the same location as the original, but with _AQ or _RQ at the end of the filename.

If you do not have v2.4, you can upgrade your software here (http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-7900ht-fast-real-timespcr-system.html).

One benefit of this conversion feature is that it allows you to convert 7900 files to the correct type to do analysis for relative quantification study, for example, if a plate is originally run as AQ instead of RQ, or if you would like to generate a standard curve quantification in the AQ if a plate was originally run as RQ.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why are there no RQ values for all the samples in my study in the 7900HT Fast Real-Time PCR System software?

The 7900HT Fast Real-Time PCR System software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have Ct values for all the wells, but the software will not calculate RQ values. If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/dataassist-software.html?icid=fr-realtimesoftware-5) instead, or use the Thermo Fisher Cloud (https://www.thermofisher.com/us/en/home/cloud.html). This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7900HT software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why do I not see any Ct or RQ values from my ddCT experiment in the 7900HT Fast Real-Time PCR System software?

In the 7900HT Fast Real-Time PCR System software, v2.3 or later, CT or RQ values are only provided in the RQ Manager software. RQ Manager is installed on the computer along with SDS v2.x.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What can I use for data analysis on the 7900HT Fast Real-Time PCR System?

For genotyping data, we recommend TaqMan Genotyper Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/taqman-genotyper-software.html?icid=fr-software-1).
For relative quantitation, we recommend ExpressionSuite Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/expressionsuite-software.html). You may also use the Thermo Fisher Cloud for either application. https://www.thermofisher.com/us/en/home/cloud.html

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What dyes can I use on the 7900HT Fast Real-Time PCR System?

The Applied Biosystems 7900HT Fast Real-Time PCR Systems use the following dye sets for calibration: FAM, SYBR, VIC, ROX, NED, TAMRA, JOE, TET dyes. Custom dyes can also be used that read between 500 nm and 660 nm, although you will have to calibrate the system first for any new dye.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

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