Tempus™ 血液 RNA 管
Tempus™ 血液 RNA 管
Applied Biosystems™

Tempus™ 血液 RNA 管

Tempus 血液 RNA 管可用于稳定和分离全血中的总 RNA,以进行基因表达分析。Tempus 血液 RNA 管的优势:•可立即一步裂解全血细胞并稳定 RNA—无需了解更多信息
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货号数量
434279250 管
货号 4342792
价格(CNY)
4,490.00
Online Exclusive
Ends: 31-Dec-2025
5,439.00
共减 949.00 (17%)
Each
添加至购物车
数量:
50 管
价格(CNY)
4,490.00
Online Exclusive
Ends: 31-Dec-2025
5,439.00
共减 949.00 (17%)
Each
添加至购物车
Tempus 血液 RNA 管可用于稳定和分离全血中的总 RNA,以进行基因表达分析。Tempus 血液 RNA 管的优势:

•可立即一步裂解全血细胞并稳定 RNA—无需 RBC 裂解或蛋白酶 K 处理
• 冷冻全局基因表达谱在室温下保持稳定长达五天;在 4°C 下保持稳定超过七天

工作原理
每支 Tempus 血液 RNA 管含 6 mL 稳定试剂。当将血液 (3 mL) 抽取至管中并与本试剂混合时,裂解几乎立即发生。稳定试剂可灭活细胞 RNase 和选择性沉淀 RNA;而基因组 DNA (gDNA) 和蛋白质仍保留在溶液中。抽取至 Tempus 血液 RNA 管中的血液可在室温下稳定保存长达五天,或在 4°C 下稳定保存至少七天。

辅助产品
当 Tempus 血液 RNA 管与 Tempus 离心 RNA 分离试剂盒(货号 4380204)配合使用时,生成的 RNA 稳定性更高且 RNA 得率更高。
仅供科研使用。不可用于诊断程序。
规格
描述血液 RNA 管
数量50 管
运输条件室温
原始材料量3 mL of Whole Blood
样品类型Whole Blood
Unit SizeEach
内容与储存
在室温储存未使用的管。

常见问题解答 (FAQ)

我正在处理血液样品并使用Tempus收集管。Tempus系统是否兼容RiboPure血液RNA纯化试剂盒?

不兼容,请遵循Tempus系统的RNA分离方法。Tempus收集管与RiboPure试剂盒不兼容。

I am doing blood work and using the Tempus collection tubes. Is the Tempus system compatible with the RiboPure-Blood RNA Purification Kit?

No, please follow the RNA isolation methods described with the Tempus system. The collection tubes are not compatible with the RiboPure kit.

What is the recommended storage temperature and stability of samples in the Tempus Blood RNA Tube?

Our general recommendations are that once blood has been collected in the Tempus Blood RNA Tubes, you can store the tube at room temperature for up to 5 days or at 4 degrees C for up to 7 days. For long term storage, the tube can be frozen at -80 degrees C for at least 1.5 years.

Do you offer the Stabilizing Reagent that comes inside the Tempus Blood RNA Tube (Cat. No. 4342792) as a stand-alone item?

We do not offer the Stabilizing Reagent that comes inside the Tempus Blood RNA Tube as a stand-alone item.

Can animal blood stored in Tempus Blood RNA Tubes or PAXgene tubes be prepared with the MagMAX for Stabilized Blood Tubes RNA Isolation Kits (Cat. Nos. 4451893 & 4451894)?

Equine whole blood has been successfully tested using this system, getting RINs around 9. Bovine blood should work similarly since there is not much of a difference between the two species for RNA isolation from whole blood, but this has not been tested. The protocol would be the same as the user guide and samples should be mixed vigorously immediately after blood is drawn into the tube for at least 10 secs, incorporating tube inversion to be sure the sample is stabilized.

引用和文献 (7)

引用和文献
Abstract
Assessment of sample collection and storage methods for multicenter immunologic research in children.
Authors:Matheson LA, Duong TT, Rosenberg AM, Yeung RS
Journal:J Immunol Methods
PubMed ID:18771669
'Multicenter studies involving both large and small centers separated by significant distances pose unique challenges to biological sample collection. The objective of this study was to evaluate protocols for determining inflammatory biomarkers that are cost and manpower efficient for handling blood destined for a sample repository. Tempus (Applied Biosystems) and ... More
Peripheral blood gene expression: it all boils down to the RNA collection tubes.
Authors:Menke A, Rex-Haffner M, Klengel T, Binder EB, Mehta D
Journal:BMC Res Notes
PubMed ID:22214347
'Gene expression profiling from peripheral blood is a valuable tool for biomarker discovery in clinical studies. Different whole blood RNA collection and processing methods are highly variable and might confound comparisons of results across studies. The main aim of the study was to compare genome-wide gene expression profiles obtained from ... More
High alpha-defensin levels in patients with systemic lupus erythematosus.
Authors:Sthoeger ZM, Bezalel S, Chapnik N, Asher I, Froy O
Journal:Immunology
PubMed ID:19191901
'Innate immunity plays a role in systemic lupus erythematosus (SLE). Our objective was to determine the levels of defensins, which are antimicrobial and immunomodulatory polypeptides, in SLE. Sera from SLE patients and healthy controls were tested for pro-inflammatory human beta-defensin 2 (hBD-2) and for alpha-defensin human neutrophil peptide 1 (HNP-1). ... More
CRY2 is associated with depression.
Authors:Lavebratt C, Sjöholm LK, Soronen P, Paunio T, Vawter MP, Bunney WE, Adolfsson R, Forsell Y, Wu JC, Kelsoe JR, Partonen T, Schalling M
Journal:PLoS One
PubMed ID:20195522
Abnormalities in the circadian clockwork often characterize patients with major depressive and bipolar disorders. Circadian clock genes are targets of interest in these patients. CRY2 is a circadian gene that participates in regulation of the evening oscillator. This is of interest in mood disorders where a lack of switch from ... More
Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation.
Authors:Asare AL, Kolchinsky SA, Gao Z, Wang R, Raddassi K, Bourcier K, Seyfert-Margolis V
Journal:BMC Genomics
PubMed ID:18847473
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. In both systems the blood is immediately lysed when collected into ... More