TaqMan™ Pri-miRNA Assay
TaqMan™ Pri-miRNA Assay
Applied Biosystems™

TaqMan™ Pri-miRNA Assay

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TaqMan Pri-miRNA Assays 对primary microRNA (pri-miRNA) 转录进行定量,这是 microRNA 生物合成通路中最上游的 RNA了解更多信息
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货号数量
4427012S (360 reactions), made to order
4427013M (750 reactions), made to order
4427014L (2900 reactions), made to order
货号 4427012
价格(CNY)
4,532.00
Each
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数量:
S (360 reactions), made to order
TaqMan Pri-miRNA Assays 对primary microRNA (pri-miRNA) 转录进行定量,这是 microRNA 生物合成通路中最上游的 RNA 分子。它们是使用较先进的生物信息学流程设计的,可为 pri-miRNA 研究提供金标准assay性能和数据质量。

高特异性—对单个基因组位点的 miRNA 转录进行定量
快速、简单且可扩展—两步 RT-qPCR 可提供高质量结果
新观点—在基因水平上测定 miRNA 表达

TaqMan Pri-miRNA Assays 对 pri-miRNA 转录的assay应用了相同的高灵敏度、卓越的特异性和广泛的线性动态范围(所有Taqman基因组assay都以这些特性而著称)。由于其高灵敏度,TaqMan Pri-miRNA Assays 仅需低至 1 ng 的加样量,即可对中度至高表达 miRNA 基因位点的表达进行量化。

设计和选择
因为尚未对所有的 pri-miRNA 转录进行映射,这些assay设计在接近 Sanger miRBase 序列储库中显示的每个茎环序列的范围内。这确保了目标转录的准确测量。每个完全公开可用的茎环都可以与 TaqMan Pri-miRNA Assay 和 TaqMan MicroRNA Assay 相匹配,从而能够独立定量分析 miRNA 成熟通路两端产生的 RNA 序列

预先配制的assay(20X;60X 预混液)
•2未标记的 PCR 引物(900 nM 每个最终 1X 反应浓度;150 nM 最终 1X 反应浓度)
• 1 FAM 染料标记的 TaqMan MGB 探针(250 nM 最终 1X 反应浓度)

仅供科研使用。不可用于诊断程序。
规格
保真度(相对于 Taq)60 X
产品规格管装
环保功能绿色可持续包装
反应次数360 次反应
产品线TaqMan™
产品类型Pri-miRNA 测定试剂盒
数量S (360 reactions), made to order
运输条件室温
足够用于360 次反应
最大浓度60X
高 GC PCR 扩增效果
PCR 方法qPCR
反应速度快速
Unit SizeEach
内容与储存
1 个含 20X(S 和 M 规格)或 60X(L 规格)预配制检测试剂(1 种探针和 2 种引物)混合物的管。

在 -15 至 -25°C 下储存。

常见问题解答 (FAQ)

Can a TaqMan Pri-miRNA Assay detect more than a single stem-loop?

TaqMan Pri-miRNA Assays are designed to detect the transcript that contains the targeted stem loop. In some instances, e.g. when stem-loop sequences are clustered together and are transcribed on the same transcript, a TaqMan Pri-miRNA Assay targeting one of the stem-loops will also detect the other stem loops in the cluster.

Do the TaqMan MicroRNA Assays detect only the mature form of a miRNA? Vice versa, do the TaqMan Pri-miRNA Assays detect only the pri-form?

Yes. The specific stem-loop chemistry of the TaqMan MicroRNA Assay and its design prevent amplification of the pri-form. Vice versa, the chemistry of the TaqMan Pri-miRNA Assays is not amenable to amplify the mature form. In particular cases, assays designed to detect mature miRNAs derived from the 3' arm of the stem loop may also detect pre-miRNA.

What endogenous control to use for pri-miRNA?

TaqMan endogenous control assays are compatible for pri-miRNA (visit the TaqMan Endogenous Control Assays web portal for more information).

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

  • High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
  • High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).


Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.