I am using the PrepSEQ Nucleic Acid Extraction Kit for Food and Environmental Testing. I am finding it very difficult to resuspend the bacterial pellet. Can you offer some tips?
This is due to the pellet being too hard. We recommend ensuring maximum resuspension of the pellet in the Lysis Buffer or Proteinase K Buffer before proceeding. Also, we recommend transferring the entire contents, including the incompletely resuspended pellet (if any), to the Lysis Plate.