Path-ID™ 多重一步法试剂盒
Path-ID™ 多重一步法试剂盒
Path-ID™ 多重一步法试剂盒
Path-ID™ 多重一步法试剂盒
Path-ID™ 多重一步法试剂盒
Path-ID™ 多重一步法试剂盒
Path-ID™ 多重一步法试剂盒
Applied Biosystems™

Path-ID™ 多重一步法试剂盒

Path-ID™ 多重一步法 RT-PCR 试剂盒设计用于多重、定量、逆转录 PCR (qRT-PCR)。针对使用 RNA了解更多信息
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货号反应次数
4428206100 次反应
4428207500 次反应
44400221000 次反应
货号 4428206
价格(CNY)
3,834.00
Each
添加至购物车
反应次数:
100 次反应
价格(CNY)
3,834.00
Each
添加至购物车
Path-ID™ 多重一步法 RT-PCR 试剂盒设计用于多重、定量、逆转录 PCR (qRT-PCR)。针对使用 RNA 样品和 TaqMan® 引物⁄探针组同时扩增多达四个靶标,对其进行了优化。以单管形式、一步法运行反应,以逆转录 RNA 并扩增您的靶标。该试剂盒包括含 Arrayscript™ 逆转录酶和 AmpliTaq Gold® DNA 聚合酶的多重酶混合物,以及缓冲液混合物。
仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7000系统,7300系统,7500 Fast 系统,7500系统,7700系统,7900 HT 系统、 AB 7900 HT 384 孔系统,StepOne™,标准模式,StepOnePlus™,标准模式
产品规格管装
热启动内置热启动
反应次数100 次反应
参比荧光染料ROX(预混)
聚合酶AmpliTaq Gold DNA 聚合酶
产品线路径-ID
产品类型一步法 RT-PCR 试剂盒
数量100 reactions
逆转录酶ArrayScript™
样品类型总 RNA,病毒 RNA,mRNA
足够用于100 次反应
检测方法引物-探针
适用于(应用)病原检测, 病毒检测
形式液体
PCR 方法一步法 RT-qPCR, 多重 qPCR
反应速度标准
Unit SizeEach
内容与储存
• 1.375 mL 2X 多重 RT-PCR 缓冲液
• 280 μL 10X 多重酶混合物,其中包括 AmpliTaq Gold 和 ArrayScript
• 1.75 mL 无核酸酶水
• 试剂盒 -20°C 储存;无核酸酶水可在 -20°C、4°C 或室温下储存

保质期至少60天(确切的有效期已印在产品和分析证书上)。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.