TaqMan™ Fast Advanced 预混液
TaqMan™ Fast Advanced 预混液
引用和文献 (3430)
Applied Biosystems™

TaqMan™ Fast Advanced 预混液

TaqMan Fast Advanced 预混液在一系列 qPCR 仪器平台上以更短的时间提供准确的定量和可靠性。即使是具有挑战性的靶标，它也能在单次反应和双重反应中提供一流的性能，包括较大的可量化范围。TaqMan Fast Advanced了解更多信息
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货号数量
44445561 x 1 mL
44445571 x 5 mL
44449632 x 5 mL
44449645 x 5 mL
444496510 x 5 mL
44445581 x 50 mL
货号 4444556
价格（CNY)
1,492.00
Each
添加至购物车
数量:
1 x 1 mL
价格（CNY)
1,492.00
Each
添加至购物车
TaqMan Fast Advanced 预混液在一系列 qPCR 仪器平台上以更短的时间提供准确的定量和可靠性。即使是具有挑战性的靶标，它也能在单次反应和双重反应中提供一流的性能，包括较大的可量化范围。TaqMan Fast Advanced 预混液包含 AmpliTaq Fast DNA 聚合酶、尿嘧啶-N-糖基化酶 (UNG)、加入 dUTP 的 dNTP、ROX 染料（被动参比）和优化的缓冲液组分。它以 2X 浓度提供。

TaqMan Fast Advanced 预混液的特点包括：
出色的性能—与标准模式下的标准混合液相比，具有更优的灵敏度、准确度、动态范围和特异性
• 专为增强台式稳定性而设计—在预装反应中，室温下可稳定长达72小时
• 针对多通路检测进行了优化—针对外源性和内源性内部阳性对照测定试剂盒的双重性进行了验证
• 缩短了在快速和标准仪器上的运行时间（<40分钟）—针对快速仪器的快速模式和标准仪器的快速循环条件进行了优化
• 无缝过渡到实验—通过 TaqMan 基因表达和 microRNA 测定试剂盒以及 TaqMan 阵列微流体卡进行了验证

出色的性能
TaqMan Fast Advanced 预混液旨在提供与标准预混液相当或更好的性能。目前已针对领先供应商的标准和快速预混液对其进行了基准测试，以帮助确保妥善提供一流的灵敏度、准确度、动态范围和特异性。TaqMan Fast Advanced 预混液的动态范围（可达7个对数级范围）是业界较广泛的。下图在一组基因表达测定中比较了 TaqMan Fast Advanced 预混液和 TaqMan Universal PCR 预混液的 Ct 值，展示了该预混液出色的灵敏度。

台式稳定性，实现高通量的处理和便利性
TaqMan Fast Advanced 预混液经过专门设计，在预装反应中，可保持长达72小时的高水平性能。此预混液具有高稳定性，可向高通量液体处理系统的用户保证第一检测板的结果与最后一个检测板的结果相仿。下图显示了在组装时（时间0）和在 30°C 下孵育72小时后运行的分析，模拟了极端的室温场景。

对于没有极高通量要求的用户，此预混液增强了稳定性，可提高工作流程的整体便利性，因为您不再受限于组装后立即运行检测板。

针对多通路检测进行了优化
我们意识到结果的可靠性至关重要。为了增加每个孔中所见结果的信心，TaqMan Fast Advanced 预混液设计使用内部阳性对照 (IPC) 为双重反应提供准确的结果。下图显示了实验靶标 β-肌动蛋白（基因名称：ACTB）的结果，其在单靶标反应和双重反应中连续稀释和扩增。

已通过 microRNA 测定验证
TaqMan Fast Advanced 预混液已经过验证，可用于多种实时 PCR 应用，包括 microRNA 测定。该配方提供了较高的特异性和动态范围，这两个较关键的性能定义了处理 microRNA 时的成功结果。下图中的数据显示了在起始模板的6个对数级范围内，PCR 具有出色的线性。

缩短在标准仪器上的运行时间
TaqMan Fast Advanced 预混液经过优化，可在快速和标准仪器配套使用，使目前拥有标准仪器的研究人员也能获得这种预混液提供的性能优势和高效性。下图展示了在快速热循环条件下，在 Applied Biosystems 7300 实时荧光定量 PCR 系统中使用 TaqMan Fast Advanced 预混液所取得令人印象深刻的结果。该预混液已经过所有 Applied Biosystems 标准实时荧光定量 PCR 仪器（7900HT、7500 和 7300 系统）的测试，无论您是否拥有快速启用的仪器，都可以确保成功。
仅供科研使用。不可用于诊断程序。
规格
保真度（相对于 Taq）2 X
适用于（设备）7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System
包括dUTP, UNG/UDG
Inhibitor Tolerance (Simple)High
多路传输能力Duplex
反应次数100 次反应
参比荧光染料ROX (Pre-mixed)
聚合酶AmpliTaq 快速 DNA 聚合酶
产品线TaqMan
产品类型Fast Advanced 预混液
数量1 x 1 mL
样品类型DNA（基因组）、cDNA
运输条件湿冰
靶标特异性cDNA or gDNA only
热稳定性72 hr., Assembled Reaction
容量1 mL
最大浓度2X
检测方法引物-探针
适用于（应用）Gene Expression, DNA Quantitation, Presence/Absence, miRNA analysis
形式液体
高 GC PCR 扩增效果高
PCR 方法qPCR
反应速度快速
Unit SizeEach
内容与储存
含有1管 (1 mL) 2X TaqMan Fast Advanced 预混液，足以进行100次反应

TaqMan Fast Advanced 预混液含有 AmpliTaq Fast DNA 聚合酶、尿嘧啶-N 糖基化酶 (UNG)、dNTP（含 dUTP）、ROX 染料（惰性参比染料）和经优化的缓冲液。

储存在 4°C 下。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all TaqMan™ Fast Advanced 预混液 FAQs

引用和文献 (3430)

引用和文献
Abstract
Microglia TREM2(R47H) Alzheimer-linked variant enhances excitatory transmission and reduces LTP via increased TNF-α levels.
Authors:Ren S,Yao W,Tambini MD,Yin T,Norris KA,D'Adamio L
Journal:eLife
PubMed ID:32579116
To study the mechanisms by which the p.R47H variant of the microglia gene and Alzheimer’s disease (AD) risk factor TREM2 increases dementia risk, we created Trem2(R47H) KI rats. Trem2(R47H) rats were engineered to produce human Aβ to define human-Aβ-dependent and -independent pathogenic mechanisms triggered by this variant. Interestingly, pre- and ... More
PARP9-DTX3L ubiquitin ligase targets host histone H2BJ and viral 3C protease to enhance interferon signaling and control viral infection.
Authors:Zhang Y,Mao D,Roswit WT,Jin X,Patel AC,Patel DA,Agapov E,Wang Z,Tidwell RM,Atkinson JJ,Huang G,McCarthy R,Yu J,Yun NE,Paessler S,Lawson TG,Omattage NS,Brett TJ,Holtzman MJ
Journal:Nature immunology
PubMed ID:26479788
Enhancing the response to interferon could offer an immunological advantage to the host. In support of this concept, we used a modified form of the transcription factor STAT1 to achieve hyper-responsiveness to interferon without toxicity and markedly improve antiviral function in transgenic mice and transduced human cells. We found that ... More
Unique maternal immune and functional microbial profiles during prenatal stress.
Authors:Antonson AM,Evans MV,Galley JD,Chen HJ,Rajasekera TA,Lammers SM,Hale VL,Bailey MT,Gur TL
Journal:Scientific reports
PubMed ID:33219314
Maternal stress during pregnancy is widespread and is associated with poor offspring outcomes, including long-term mental health issues. Prenatal stress-induced fetal neuroinflammation is thought to underlie aberrant neurodevelopment and to derive from a disruption in intrauterine immune homeostasis, though the exact origins are incompletely defined. We aimed to identify divergent ... More
A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons.
Authors:Xu H,Iyer N,Huettner JE,Sakiyama-Elbert SE
Journal:Stem cell research & therapy
PubMed ID:26555777
INTRODUCTION: Spinal V3 interneurons (INs) are a commissural, glutamatergic, propriospinal neuron population that holds great potential for understanding locomotion circuitry and local rewiring after spinal cord injury. Embryonic stem cells hold promise as a cell source. However, the inevitable heterogeneity resulting from differentiation protocols makes studying post-mitotic stem cell-derived neuron ... More
Electrospun biomaterial scaffolds with varied topographies for neuronal differentiation of human-induced pluripotent stem cells.
Authors:Mohtaram NK,Ko J,King C,Sun L,Muller N,Jun MB,Willerth SM
Journal:Journal of biomedical materials research. Part A
PubMed ID:25524598
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